Use of sexed semen in conjunction with in vitro embryo production is a potentially efficient means of obtaining offspring of predetermined sex. For thousands of years, livestock owners have desired a methodology to predetermine the sex of offspring for their herds. The ability to sort individual sperm cells into viable X- and Y-chromosome-bearing fractions made producers' sex selection dreams r...

This study examined DNA damage and postthaw motility of white-tailed deer sperm (n = 28) before and after sex selection and conventional sorting using MoFlo XDP SX flow cytometry. Semen samples from the same individuals were treated in 4 different ways: 1) chilled-extended sperm samples (without glycerol); 2) cryopreserved conventional samples, samples directly cryopreserved after the addition ...

Background: Newborn gender ratio is one of the most important factors in livestock industry. This ratio has affected on economic condition and controls sex-linked genetically transferred diseases. Offspring sex ratio is affected by such various factors. One of these factors is the Y to X bearing sperm ratio in normal semen that is called primary sex ratio, season and individual differences are ...

Sex preselection is a subject that has held man’s attention for generations. The most effective way to achieve sex predetermination is to resolve X and Y chromosome bearing sperm populations. One of the most reliable methods of sorting spermatozoa is flow cyto-fluorimetric analysis, which is based on the difference in the DNA content of the X and Y sperm populations. The difference in the DNA c...

Here we present kinetics data from bovine sex-specific embryo development. Embryos were originated using sex-sorted semen from three different Nelore bulls, and semen from the same batch was used for X-and Y-chromosome spermatozoa sorting. Data was obtained for six time points (24, 48, 96, 120, and 144 h.p.i.). Analyses for each bull׳s embryos (1, 2 and 3) is presented for female and male group...

BACKGROUND Flow cytometric sorting can be used to separate sperm based on sex chromosome content. Differential fluorescence emitted by stained X- vs. Y-chromosome-bearing sperm enables sorting and collection of samples enriched in either X- or Y-bearing sperm for use to influence the likelihood that the offspring will be a particular sex. Herein we report the effectiveness of flow cytometric so...