this study was carried out to investigate the morphological and anatomical aspects of somatic embryogenesis in date palm. lateral bud and shoot tip explants excised from young offshoots were cultured on ms medium with 2,4-d. somatic embryogenesis was in-duced by transferring the calli produced on the same medium without hormones. micro-tome sectioning of paraffin-embedded specimens was carried ...

abstract somatic embryogenesis is a new approach to in vitro plant propagation. this technique is based on stem cell obtain and its differentiation to a complete plant. this is affected by components of media culture. so, the effects of ms, b5 and nl media on carrot petiole somatic embryogenesis investigated via histological experiment. to do this, carrot petioles (with 1 cm dimension) cultured...

many factors may influence the efficiency of somatic embryogenesis. this capability may be differ with regard to media compositions, genotype, tissue, organ ontology and the stage of differentiation. the effects of five salicylic acid concentrations (0, 25, 50, 75 and 100 mm) on different stages of carrot somatic embryogenesis were studied using petiole and root secondary phloem explants as sta...

somatic embryogenesis is a new approache to plant mass in vitro propagation. this method influences by elements and their components in medium. in order to investigate the effects of different media and it,s role on somatic embryogenesis of carrot, a cbd experiment with four replications (including 12 treatments) was conducted. to do this, carrot petioles (with 1 cm dimension) in induction phas...

to explore the potential for in vitro rapid regeneration of fagonia indica burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on ms medium which supplemented with 1.0-5.0 µm 2,4-d. callus was initiated from cotyledon explant on ms medium supplemented in all concentrations of 2,4-d investigated. 2,4-d was more effective than iaa and naa for cal...

somatic embryogenesis was initiated in c. sativus l. from shoot meristems on ls medium containing bap (2×10-5 m)+naa (2×10-5 m). various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. matured embryos coul...

objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...

Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

The present study was conducted in two different experiments based on complete randomized design to evaluate the effects of calcium (0, 1, 2, 4 mM) and magnesium (0, 1.02., 2.04, 4.08 mM) on carrot petiole somatic embryogenesis in B5 medium. A difference on somatic embryogenesis was observed among different levels of calcium and magnesium. The number of globular embryos in medium with low magne...

one of the important methods of plant propagation in large scale so as genetic manipulation and artificial seed production is somatic embryogenesis technique. the obtained results from this methods related to different factors including condition of culture medium. therefore, the aim of this study conduction were determining the role of molybdenum during carrot petiole somatic embryogenesis. at...