somaclonal variation is one of the possible sources of variation in plant breeding. to evaluate the usefulness of somaclonal variation for creating variation in iranian cultivars of wheat, proteins, enzymes and chromosome number of regenerated and seed-produced plants of wheat (alamout, hyrmand and maroon) were studied. plantlets obtained from the immature embryos and seed culture were collecte...

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy INDIRECT SHOOT ORGANOGENESIS AND SELECTION OF SOMACLONAL VARIATION IN Dieffenbachia 11 By Xiuli Shen May 2007 Chair: Michael E Kane Cochair: Jianjun Chen Major: Horticultural Science A series of experiments were conducted to investigate ...

BACKGROUND Somaclonal variation generally occurs in plants regenerated from tissue culture. However, fundamental issues regarding molecular characteristics, mutation rates and mutation spectra of plant somatic variation as well as their phenotypic relevance have been addressed only recently. Moreover, these studies have reported highly discrepant results in different plant species and even in t...

In this study, calli were obtained from in vitro grown potato stem or leaf segments cv. Cosima on MS medium containing 2,4-D, NAA, kinetin and yeast extract. After three subcultures, well grown calli were exposed to UV-C radiation with approximately 6.24 μmol photon/m2/s for 30 min three times at an interval of 2 weeks. Somaclonal variation induced by UV-C radiation was revealed by 5 out of 28 ...

Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using culture conditions that allow moderate cell proliferation. In view of true-to-type large-scale propagat...

BACKGROUND The recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. This is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. This study investigates a new strategy of assessing mul...

effects of culture medium containing hormonal compositions on phenotypic and chromosomal variations of sugarbeet cultured tissues were examined. experiments were carried out in different nacl levels. cytological studies were conducted on calli originated from suspension culture. hypocotyl of seven day old seedlings of the two diploid lines were cultured in ms medium containing hormonal composit...

In this study Artemisia amygdalina a critically endangered medicinal plant endemic to Kashmir Himalayas was grown under in vitro conditions. ISSR markers were used to study the somaclonal variation at the genetic level. Two ISSR-primers (CAC)3 GC and (ACTG)5 were used, two additional DNA bands (0.15kb and 0.2kb) were observed respectively in tissue cultured Artemisia amygdalina. Direct regenera...