Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...

three experiments were conducted in tissue culture and biotechnology laboratory of horticulture department of mohaghegh ardabili university in 2012. for the regeneration of plant from seed, different concentrations of naoh (5, 10, 15, 20 m) and various scarification methods with sandpaper (soft scarification, medium scarification and hard scarification) were used based on completely randomized ...

Background: Sodium caseinate is a rich source of protein and minerals originating from animals. Numerous food and non-food products are made from sodium caseinate. The present study investigated the chemical components (moisture, crude protein, ash, and soluble crude protein) of sodium caseinate prepared by different acids and drying techniques. Methods: A completely randomized factorial desig...

chrysanthemum cut flower is not sensitive to ethylene and its vase life depends on vascular blockage. in order to evaluating of effect of antibiotics and essential oils on the vase life and quality characteristics of chrysanthemum cut flower an experiment carried out based on a rcd with 10 treatments: artemisia at 3 levels (10, 30 and 50 %), amoxicillin at 3 levels (100, 200 and 300 mg l-1), ri...

the oil and protein content of three varieties of water melon seeds namely, chillian black, dixy queen and charlston gray were determined. the soluble proteins were studied and measured by paper chromatography and amino acid analyzer. the results indicated that oil content of the seeds was 43, 53 and 45% and protein content was 27, 23, and 34% respectively. the soluble proteins contained all th...

Net release of degraded N as NH3 and total AA plus microbial protein synthesis, quantified from incorporation of 15NH3 into microbial protein, was used to estimate the rate and extent of in vitro degradation of protein fractions isolated from alfalfa hay and silage. Seven proteins (casein, alfalfa hay, alfalfa silage, extracts from alfalfa hay and silage, and residues from alfalfa hay and silag...

Total soluble solid (TSS) content is commercially used as fruit quality index because of its high positive correlation with sugar content. In the present study, The effect of foliar application of naphthalene acetic acid (NAA) with different concentrations (0, 25, 50, and 100 mg.L-1) at 4 true leaf and fruit set stages and plant training (pruning and thinning) on TSS and soluble sugar (i.e. suc...

Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

in order to study the yield and forage quality of intercropping barley (hordeum vulgare) and mustard (sinapis alba) in different planting dates, a factorial experiment based on randomized completely block design with three replications was carried out at farm research station, gonbad -e- qabus university. factors were included as three planting dates (15 november, 6 and 20 december 2006) and fo...