The properties and subcellular distribution of phosphatidate phosphatase (EC 3.1.3.4) from adipose tissue have been investigated. The enzyme was assayed using both aqueous phosphatidate and membrane-bound phosphatidate as substrates. When measured with aqueous substrate, activity was detected in the mitochondria, the microsomes, and the soluble fraction. Mg(2+) at low concentration stimulated t...

Understanding substrate binding and product release in cytochrome P450 (CYP) enzymes is important for explaining their key role in drug metabolism, toxicity, xenobiotic degradation and biosynthesis. Here, molecular simulations of substrate and product exit from the buried active site of a mammalian P450, the microsomal CYP2C5, identified a dominant exit channel, termed pathway (pw) 2c. Previous...

In the present study, packaging of a greenhouse-grown tomato on the culture medium containing 0, 25, 50, 75 and 100% compost tea under modified atmosphere: MA (88%N2 + 8%CO2 + 4%O2) in polyethylene and nanosilicon-polyethylene bags was studied at 5°C for 30 days. Several quality parameters such as moisture content, pH, total soluble solids (TSS), titratable acidity (TA), Vitamin C content, text...

The hemoglobin dehaloperoxidase (DHP), found in the coelom of the terebellid polychaete Amphitrite ornata, is a dual-function protein that has the characteristics of both hemoglobins and peroxidases. In addition to oxygen transport function, DHP readily oxidizes halogenated phenols in the presence of hydrogen peroxide. The peroxidase activity of DHP is high relative to that of wild-type myoglob...

A statistical model, using Artificial Neural Networks (ANN), has been developed for the aerobic suspended growth Wastewater Treatment (WWT) plant. The paper presents the way ANN model has been designed and trained. The emerged recurrent ANN model has been used to perform WWT control using Model Predictive Control (MPC) algorithm. Model Predictive Control of the WWT soluble substrate and dissolv...

The activity of cyclic 3':5'-nucleotide phosphodiesterase (PDE) (EC 3.1.4.17) was measured in cultured normal and neoplastic rat mammary epithelium. Total PDE activity in normal cells was 1.6 to 6 times higher than that in tumor cells over a concentration range of 0.01 to 1 mM cyclic adenosine 3':5'-monophosphate. PDE activity was distributed between the low-speed (4000 x g) particulate and sup...

1. The properties of rat brain lysophospholipase were investigated. Three subcellular fractions were employed: a particulate preparation which sedimented at 26,000 X g, a microsomal preparation which sedimented at 100,000 X g, and the supernatant of the above. The soluble enzyme represented only a small fraction of the total activity; it was further purified by treatment with protamine and ammo...

The influence of J1/tenascin adsorbed to polyornithine-conditioned plastic (substrate-bound J1/tenascin) and J1/tenascin present in the culture medium (soluble J1/tenascin) on neurite outgrowth was studied with cultured single cells from hippocampus and mesencephalon of embryonic rats. Neurons at low density grew well on J1/tenascin substrates and extended neurites that were approximately 40% l...