After separation of whole proteins of chick neural retina by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), a number of glycoproteins can be detected by staining the gels with 125I-labeled wheat germ agglutinin (WGA) and other lectins. The glycoprotein patterns show both quantitative and qualitative changes between days 7 and 13 of development. Some of these...

Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl ...

A chitinase has been obtained in milligram amounts from wheat germ by affinity chromatography on chitin followed by chromatography on Sephadex G-50. The purified enzyme was free from wheat germ agglutinin and showed a single peak on sodium dodecyl sulfateacrylamide gel electrophoresis. Isoelectric focussing on acrylamide gel slabs gave rise, however, to several bands, with isoelectric points ra...

the anionic surfactant sodium dodecyl sulfate (sds) was degraded by novel strain ofpseudomonas aeruginosa kgs under accession no. jq328193, which was isolated from carwash wastewater. the purpose of this research was to study different optimization conditionsrequired for enhancing the biodegradation of sodium dodecyl sulfate p. aeruginosa kgs.influence of different physicochemical factors such ...

Fractions enriched in Golgi membranes were prepared from rat liver by sucrose gradient ultracentrifugation. These enriched membranes were further subfractionated on the basis of their solubilities in EGTA, 150 mM sodium carbonate, pH 11.5, sodium deoxycholate, Triton X-100, or sodium dodecyl sulfate. This led to isolation of peripheral, luminal, and integral membrane proteins of the Golgi-enric...

Alterations in cell surface proteins and glycoproteins may play a key role in determining the metastatic behavior of tumor cells. The cell surface proteins of a series of related murine colon cancer cells selected in an animal model for colon cancer metastasis (R. S. Bresalier et al., Cancer Res., 47:1398-1406, 1987) were therefore compared by a variety of biochemical methods. Lactoperoxidase-c...

Alterations in cell surface proteins and glycoproteins may play a key role in determining the metastatic behavior of tumor cells. The cell surface proteins of a series of related murine colon cancer cells selected in an animal model for colon cancer metastasis (R. S. Bresalier et al., Cancer Res., 47: 1398-1406, 1987) were therefore compared by a variety of biochemical methods. Lactoperoxidase-...

Neonatal rat lung prolyl hydroxylase was synthesized in a cell-free system. Total polysomes were isolated from neonatal rat lung homogenates after Triton X-100 and deoxycholate solubilization. The polysomes were then translated in a wheat germ lysate in the presence of tritiated leucine. The radioactive product was precipitated with antibody to rat prolyl hydroxylase and co-precipitated with pr...

Alterations in cell surface proteins and glycoproteins may play a key role in determining the metastatic behavior of tumor cells. The cell surface proteins of a series of related murine colon cancer cells selected in an animal model for colon cancer metastasis (R. S. Bresalier et al., Cancer Res., 47:1398-1406, 1987) were therefore compared by a variety of biochemical methods. Lactoperoxidase-c...

The addition of O-linked N-acetylglucosamine (GlcNAc) to the major nuclear pore complex glycoprotein p62 was examined. Expression of the rat p62 cDNA in transfected monkey cells was detected using a rat p62-specific antipeptide antiserum and two previously described nuclear pore-specific monoclonal antibodies which require O-linked GlcNAc for binding. Although the p62 cDNA was predicted to enco...