BACKGROUND Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the p...

The formation mechanism and wear behavior of a gradient nanostructured (GNS) Inconel 625 alloy were investigated using SEM, TEM ball-on-disc sliding tester. results show that surface mechanical grinding treatment (SMGT) induced an approximately 800 μm-deep microstructure, consisting nano-grained, nano-laminated, nano-twined, severely deformed layers, which resulted in reduced micro-hardness fro...

Sperm-mediated gene transfer (SMGT) might become the most efficient and cost effective technique to generate transgenic animals, which will significantly increase their application in biomedical research and in commercial production. Despite some successes, the technique has remained controversial for almost 20 years and despite number of studies the reasons for poor reproducibility of this pro...

sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous dna. in the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton x100 and dmso, on exogenous dna uptake by sheep spermatozoa and...

A transgenic animal as originally defined by Gordon and Ruddle (1981), is an organism carrying recombinant molecules that were intentionally introduced by human intervention. Classically this entailed the stable integration of the transgene within the host animal’s genome and its transmission to progeny through normal breeding programmes. Transgenic chicken holds great promise in basic biologic...

Throughout the last decades developments on desalination field have been focused energy consumption and costs reduction. However, water recovery brine disposal are becoming a matter of concern to industry. In this work, Zero Liquid Discharge (ZLD) unit coupled with Solar Micro Gas Turbine (SMGT) system is presented address, among others, challenges mining industry in remote areas, particular, f...

Intracytoplasmic sperm injection-sperm-mediated gene transfer (ICSI-SMGT) is a useful tool for the production of transgenic mice but is still rather inefficient in farm animals. In the current study, we evaluated the effect of the sperm treatments on the efficiency for producing enhanced green fluorescent protein (EGFP)-expressing pig embryos by ICSI-SMGT. Four different sperm treatments were a...