نتایج جستجو برای: sirna 4tr

تعداد نتایج: 21615  

Journal: :iranian biomedical journal 0
chinh chung doan ngoc trung doan quang huy nguyen minh hoa nguyen minh si do van dong le

background: kinesin spindle protein (ksp) plays a critical role in mitosis. inhibition of ksp function leads to cell cycle arrest at mitosis and ultimately to cell death. the aim of this study was to suppress ksp expression by specific small-interfering rna (sirna) in hep3b cells and evaluate its anti-tumor activity. methods: three sirna targeting ksp (ksp-sirna #1-3) and one mismatched-sirna (...

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2016
D Conte A Tessitore K Smiley C Thomas TG Favero

This study aimed to analyse live and stoppage time phases, their ratio, and action played on half and full court in college basketball games. Differences were assessed for the entire games and between halves. Moreover, differences of the live/stoppage time ratio were analysed between games and game-based conditioning drills. Ten games as well as fifteen defensive, fourteen offensive and six scr...

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ژورنال: :پژوهش های آسیب شناسی زیستی 0
zahra deilami khiabani assistant professor, department of microbiology, faculty of basic sciences, zanjan branch of islamic azad university, zanjan, iran mehdi banan assistant professor, genetics research center, university of social welfare and rehabilitation sciences, tehran, iran ali mohammad asgharian assistant professor, department of biology, faculty of basic sciences, tonekabon branch of islamic azad university, mazandaran, iran jalal gharesouran instructor, department of genetics, faculty of medicine, tabriz university of medical sciences, tabriz, iran hossein najmabadi professor, genetics research center, university of social welfare and rehabilitation sciences, tehran, iran

هدف: پروتئین creb یک فاکتور مهم پایین دست بسیاری از مسیرهای علامتی به شمار می رود. با طراحی sirna کارامد برای ژن creb می توان مسیرهای علامتی بسیاری از داروها را در سلول های مختلف به ویژه سلول k562 بررسی نمود. در این تحقیق میزان مهار بیان ژن creb با به کارگیری دو sirna مختلف برای این ژن بررسی شد. مواد و روش ها: طراحی sirna براساس معیار reynolds انجام گرفت. سلول های k562 با روش لیپوفکشن با sirna ...

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ژورنال: مجله پزشکی ارومیه 2019
Abedi Gaballu, Fereydoon, Dehghan, Gholamreza, Mansoori, Behzad,

Background & Aims: The use of siRNA to silence gene expression is increasingly expanding today. The aim of this study is to bioinformatically and experimentally investigate the inhibition of the HMGA2 gene and its corresponding effects on downstream genes expression rate in MDA-MB-231 cancer cell treated by shRNA and siRNA specific to HMGA2. Materials & Methods: To perform this bioinformatic a...

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ژورنال: مجله تازه های بیوتکنولوژی سلولی - مولکولی 2012
قنادی مراغه, محمد, تقی خانی, محمد , فتحی, مجتبی, یاوری, کمال,

سابقه و هدف: اثر خاموش سازی ژن با siRNA به طور قابل توجهی به یک روش مناسب جهت عرضه آن به سلول ها بستگی دارد. در پژوهش حاضر، اثر لیپوزوم ها در انتقال کمپلکس siRNA - 177Lu به سلول های سرطانی کولون به طور کمی مورد مطالعه قرار گرفته است.مواد و روش ها: siRNA با p-SCN-Bn-DTPA کونژوگه و با لوتسیم نشاندار شد. کمپلکس های siRNA - 177Lu پس از تخلیص با ستون های Vivaspin و سفادکس G25، به منظور تعیین در صد خ...

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Journal: Iranian Journal of Medical Sciences 2012
Mozhgan Rasti, Rita Arabsolghar

Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...

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Journal: :iranian journal of allergy, asthma and immunology 0
masoumeh firouzi department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran and tissue repair lab, institute of biochemistry and biophysics, university of tehran and research centre for neural repair, university of tehran farzaneh sabouni department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran abdolkhaleg deezagi department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran zahra hassannejad pirbasti tissue repair lab, institute of biochemistry and biophysics, university of tehran and research centre for neural repair, university of tehran fatemeh poorrajab tissue repair lab, institute of biochemistry and biophysics, university of tehran vafa rahimi-movaghar sina trauma and surgery research center, tehran university of medical sciences, tehran, iran

the p75 pan-neurotrophin receptor (p75ntr) plays a pivotal role in linking the immune system with the nervous system. p75ntr is required for the development of several characteristic features of allergic asthma. also p75ntr upregulated by reactive schwann cells after peripheral nerve injury. moreover p75ntr and rhoa play a critical role in the regulation of apoptosis. to determine whether the d...

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Journal: Research in Molecular Medicine 2013
Abolghasem Ajami, Alireza Rafiei, Eshrat Gharaee, Fatemeh Rahbarizadeh, Mohammad bagher Hashemi Sooteh, Mohammad Soltany-Rezaee-Rad, Negar Mottaghi-Dastjerdi, Saeid Abediankenari,

Background: RNAi (RNA interference) is a new strategy in gene therapy and biotechnology which provides new promises in the treatment of different diseases such as cancer and viral diseases. CCND1 which is a key gene in cell cycle is amplified and over expressed in esophageal cancer. The objective of this study was production and siRNAs for CCND1, the key gene in cell cycle. Materials and Metho...

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Journal: Iranian Biomedical Journal 2012
Ali Akbar Fallahi, Fatemeh Noorbakhsh, Ladan Teimoori-Toolabi , Maryam Moazeni, Mohammad Reza Khorramizadeh, Sassan Rezaie,

Background: The most important virulence factor which plays a central role in Candida albicans pathogenesis is the ability of this yeast to alternate between unicellular yeast and filamentous hyphal forms. Efg1 protein is thought to be the main positive regulating transcription factor, which is responsible for regulating hyphal-specific gene expression under most conditions. ALS3 is one of the ...

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پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده علوم 1393
فاطمه جلالی, علی خدادادی, حمید گله داری,

پیش زمینه: بیان بیش از حد bcl-2 به طور مکرر در انواع مختلفی از سرطانها مشاهده شده و یکی از مارکرهای پیش آگاهی در سرطان پستان می باشد. نقش دوگانه bcl-2 در مهار هر دو مسیر مرگ سلولی آپوپتوز و اتوفاژی دلیل مهم انتخاب آن به عنوان یک هدف درمانی ایده ال می باشد. بنابراین، مورد هدف قرار دادن نشانگر bcl-2 ممکن است به عنوان یک استراتژی انتخابی برای بهبود اثر بخشی درمان و غلبه بر مقاومت دارویی برای شیمی ...

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