Synthetic regulatory proteins such as tetracycline (tet)-controlled transcription factors are potentially useful for repression as well as ectopic activation of endogenous genes and also for probing their regulatory mechanisms, which would offer a versatile genetic tool advantageous over conventional gene targeting methods. In this study, we provide evidence supporting this concept using Cd4 as...

This study is concern about the behavior of a diesel generator sets in terms sound pressure level and drop. Numerical had been carried out by using simulation from ANSYS software to obtain drop across duct silencer sets. Fluent Harmonic Responses was used perform on silencer. The design 3-dimensional model consists splitter rockwool as its porous medium. Observation focused along process detail...

Negative regulation of cytokine gene transcription is an important mechanism in maintaining homeostasis of immune function. In this study, we characterized a silencer element in the human interleukin-3 gene promoter that is responsible for the cell-specific expression of interleukin-3. This silencer activity was proposed to be mediated by an unidentified nuclear inhibitory protein (NIP). In thi...

Significant potential for acoustic metamaterials to provide a breakthrough in sound attenuation has been unlocked recent times due advancements additive manufacturing techniques. These materials allow the targeting of specific frequencies attenuation. To date, have not demonstrated commercial automotive silencer performance enhancement. A significant obstacle practical use is need low cost and ...

Silencing of retrovirus vectors poses a significant obstacle to genetic manipulation of stem cells and their use in gene therapy. We describe a mammalian silencer blocking assay using insulator elements positioned between retrovirus silencer elements and an LCRbeta-globin reporter transgene. In transgenic mice, we show that retrovirus silencers are blocked by the cHS4 insulator. Silencer blocki...

We analyzed the activity of the enhancer, the promoter and the silencer of the human CD4 gene during T cell development using transgenic mice. Immunofluorescence studies on thymic populations of mice carrying transgenes in various combinations of these regulatory DNA elements revealed that thymocytes control the CD4 gene in a different manner than mature peripheral T lymphocytes. The 5'-positiv...

Marine diesel engine is essential on a ship, but emits extremely loud noise harmful to human hearing. Therefore, it mandatory use silencers reduce the venting of marine engine. An advanced silencer with new acoustical element curved tube, along perforated holes, presented suppress wave. To predict silencer's performance for high-order waves, finite model was built using COMSOL. The influence re...

Combining in vitro DNA binding studies and functional transcription assays in the Xenopus oocyte, we have tested the presence and functional state of transcription factors controlling the interleukin-2 (IL-2) promoter through the NFAT binding site. In naive T-helper cells, the IL-2 gene is repressed by a silencer. After first mitogenic stimulation, this silencer becomes undetectable while an ac...

The pancreatic elastase I gene (ELA1) is selectively transcribed to high levels in pancreatic acinar cells. Pancreatic specificity is imparted by a 100-base pair enhancer that activates transcription in beta-cells of the islets of Langerhans as well as in acinar cells. Adjacent to the enhancer is a silencer that renders transcription specific to acinar cells by selectively suppressing the inher...