abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

results pcslab is a weak promoter responding to come and its binding appears to be negative cooperative. although pcslab is partially controlled by comcde, it can respond to comcde regulation. supported by the obtained molecular evidence, cslab acts as a stabilizer of comcde signal on the patterns of its expression. conclusions pcslab is partially controlled by comcde. cslab is a stabilizer of ...

urokinase plasminogen activator receptor (upar) and its ligands play a major role in many tumors by mediating extracellular matrix degradation and signaling cascades leading to tumor growth, invasion and metastasis. recently we introduced upar decapeptide antagonist with cytotoxic effect on mda-mb-231 cell line. in this study we assessed the alteration in upar downstream signaling following tre...

in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...

Single chain Fragment variable (scFv) is an antibody fragment consisting variable regions of heavy and light chains. scFvs enhance their penetrability into tissues while maintaining specific affinity and having low immunogenicity. Insoluble inclusion bodies are formed when scFvs are expressed in reducing bacterial cytoplasm. One strategy for obtaining functionally active scFv is to translocate ...

administering growth hormone increases growth rate of cultured fish. the aim of this study was isolation and synthesize of persian sturgeon gh cdna. the total rna was extracted from pituitary gland of persian sturgeon and cdna was synthesized. the full-length cdna sequence of persian sturgeon contains a 645 nucleotide open reading frame, which encoding a 214 amino acid residues. the position of...