Y-short tandem repeats (STRs) are located in the nonrecombining part of the Y-chromosome. Its genetic characteristics are helpful to paternity testing. The male lineage can be pursued over several generations. Since an overwhelming majority of rape or sexual assault cases involve male suspects, these markers proved to be very useful with mixed stains. Routine casework performed in our laborator...

Background: Quantitative fluorescence-polymerase chain reaction (QF-PCR) is an inexpensive and accurate method for the prenatal diagnosis of aneuploidies that applies short tandem repeats (STRs) as a chromosome-specific marker. Despite its apparent advantages, QF-PCR is not applicable in all cases due to the presence of uninformative STRs. This study was carried out to investigate the efficienc...

INTRODUCTION All eukaryotic genomes contain regions of simple repetitive DNA, called short tandem repeats (STR*) or microsatellites, which consist of tandem repeats of a small number of bases (1-3). The number of repeats at a STR locus can be highly variable among individuals, resulting in length polymorphisms that can be detected by relatively simple PCR-based assays. Thousands of these highly...

The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR f...

Microsatellites or Short Tandem Repeats (STRs) are loci with alleles composed of tandemly repeated short DNA sequences of 2-7 base pairs in length. In the present study, the polymorphism STR loci CODIS were analyzed in 40 people with a history of crime using multiplex PCR. The 6 STRs loci included TH01, TPOX, D16S539, D18S51, CSF1PO and FGA. The result showed that the highest PD and heterozygos...

The dynamics of microsatellite, or short tandem repeats (STRs), is well documented for long, polymorphic loci, but much less is known for shorter ones. For example, the issue of a minimum threshold length for DNA slippage remains contentious. Model-fitting methods have generally concluded that slippage only occurs over a threshold length of about eight nucleotides, in contradiction with some di...

Although the utility of short tandem repeats on the Y-chromosome (Y-STRs) has long been recognized and leveraged in forensics, genealogy and paternity testing, the bulk of these applications have relied on only a few dozen loci identified as having remarkably high mutation rates. Recent efforts have expanded the set of Y-STRs with known mutation rates to two hundred markers, but the limited thr...

In northeast Argentina, different Amerindian communities share territory and history with settlers, mainly Europeans. Due to miscegenation, the current Argentinean population has a particular structure that can be described through X chromosome variation. The objectives of this study were describe variation 10 X-chromosome short tandem repeats (X-STRs) in urban populations regions known as Gran...

BACKGROUND Polymorphic Y chromosome short tandem repeats (STRs) have been widely used in population genetic and evolutionary studies. Compared to di-, tri-, and tetranucleotide repeats, STRs with longer repeat units occur more rarely and are far less commonly used. PRINCIPAL FINDINGS In order to study the evolutionary dynamics of STRs according to repeat unit size, we analysed variation at 24...

The 11 Y-chromosomal short tandem repeats (STRs) included in the Promega Corporation PowerPlex Y System (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS437, DYS438 and DYS439) were typed in three ethnic groups ("Andalusians", Berber and Arab) and one cosmopolitan population (Tunis) from Tunisia, summing up 247 individuals, and 139 different haplotypes. Focusing the analysi...