In vitro production of camptothecin (CPT) was achieved by the establishment of multiple shoot and root cultures of Ophiorrhiza rugosa Wall. var. decumbens (Gardn. ex Thw.) Deb & Mondal. The maximum multiple shoots were obtained in Murashige and Skoog (MS) medium supplemented with 5mg/l N-benzyl amino purine (BA) along with 0.5mg/l naphthalene acetic acid (NAA). Optimal root generation was obser...

this research was aimed to investgate the effect of light quality on micropropagation of pear rootstock oh×f333. first, single nodes explants were cultured in modified ql medium containing ba at four levels (0, 0.5, 1, and 2 mg/l-1) and iaa at three levels (0, 0.1, and 1 mg/l-1) and were evaluated for shoot proliferation. then, plantlets subcultured in modified ql medium containing 1 mg/l-1 ba ...

In vitro raised shoots of Plumbago rosea L. were infected with A4 strain of Agrobacterium rhizogenes to initiate hairy root formation, which produced 3.0±0.33 hairy roots per incision on explants in 20 d incubation. Southern blot analysis confirmed the integration of T-DNA into the genome of the roots. The hairy roots were cultured on MS agar medium supplemented with 2.0 mg/L BAP to induce the ...

2Botany Department, San Diego State University, San Diego, California 92182 Abstract: Purpose of this study was to determine the balance between root and shoot growth in chaparral shrubs. Under controlled conditions with ample water and nutrient supplies, growth of roots and shoots continued exponentially for 5 months. In contrast, in the field shoots had flushing periods in the spring, lasting...

Analysis of extracts obtained from shoots, roots and exudates of Brassica alba revealed the presence of 3,5,6,7,8-pentahydroxy-4'-methoxy flavone in shoots, as well as 2',3',4',5',6'-pentahydroxy chalcone and 3,5,6,7,8-pentahydroxy flavone in roots and exudates. Apigenin was also found in the shoots and roots, but not in the root exudates.

in order to study the effect of soil compaction on root and shoot growth of wheat plants (shahriar variety), a greenhouse pot experiment was conducted in guilan university. the study design was factorial with completely randomized in three replications. treatments included three compaction levels; (normal compaction, compaction of ten percent and twenty percent) and two type of soil texture (cl...

Effects of various culture media and growth regulators on micropropagation of cherry rootstock, M × M60 were investigated in the present study. Stem cuttings of the current season growth were taken from mature stockplants and cultured on the establishment medium after disinfection. After four weeks, elongated shoots were cut and subcultured on the proliferation media including MS, DKW, QL and M...

Brassinosteroids (BRs) are steroidal phytohormones that regulate various physiological processes, such as root development and stress tolerance. In the present study, we showed that brassinolide (BL) affects potato root in vitro growth in a dose-dependent manner. Low BL concentrations (0.1 and 0.01 μg/L) promoted root elongation and lateral root development, whereas high BL concentrations (1-10...

Rmsl i s one of the series of five ramosus loci in pea (Pisum sativum 1.) in which recessive mutant alleles confer increased branching at basal and aerial vegetative nodes. Shoots of the nonallelic rms l and rms2 mutants are phenotypically similar in most respects. However, we found an up to 40-fold difference in root-sap zeatin riboside ([9R]Z) concentration between rms l and rms2 plants. Comp...