The aim of the present study was to investigate the importance of the preculture conditions on the survival of apple (Malus domestica Borkh., cv. Romus4 and rootstock M106) shoot tips cryopreserved using a combined droplet vitrification procedure. Preculture was critical for the survival of apple shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, s...

Mixed fluorescent and incandescent light increased growth and sink strength of the uppermost young shoot of rose plants (Rosa hybrida cv. Marimba) in comparison to pure fluorescent light. This was manifested by increased apical dominance. Monochromatic low-energy red light, given by means of optic fibers for 24 hours to shoot tips that had been previously darkened for 5 days, increased the tran...

Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequenc...

In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. esculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were pre...

Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...

Trehalose, an a,a-diglucoside, induced a rapid blackening and death of shoot tips of Cuseuta reflexa (dodder) cultured in vitro. The onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of Cuscuta was supplied with trehalose. The rate of trehalose uptake or its accumulation in the tissue was not affected by sugar cofeeding. The levels of total and re...

tissue culture is an attractive alternative for plant propagation. micropropagation is a technique to ensure a constant and uniform source of ornamental plants. matthiola incana is an important ornamental species mainly cultivate by seed. matthiola incana seeds were germinated on solid ms medium without plant growth regulators. shoot proliferation and root formation are possible using kinetin (...

A Russian knapweed (Acroptilon repens) shoot culture system, initiated from shoot tip culture, was used to generate a source of host plant tissue for the rearing of the nematode Subanguina picridis, a biocontrol agent for Russian knapweed. Young shoots growing on solid B5G medium in petri dishes developed galls on leaves, petioles, and shoot tips 7 days after release of 50 nematodes onto the su...