Rapid in vitro multiplication of shoots has been described using capitular sections and leaf explants in Gerbera jamesonii. Three types of basal media regimes were used including MS and two of its modified forms⎯MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular s...

The present research work involves shoot formation, their multiplication and rooting in carnation Dianthus caryophyllous. For shoot formation both apical and nodal meristems were used. MS medium containing BAP alone or in combination with kinetin was tested. Best shoot formation response was obtained after 6 days of inoculation from apical meristem and after 7 days of inoculation from nodal mer...

Shoot tips of two fig cultivars (Conadria and Black Mission) proliferated in vitro, were cultured on modified MS medium supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The present investigation aimed to investigate the effect of different medium salt strengths (Double, full, half and quarter) and different sources of carbon (sucrose and fructose) at different concentrations (0.1 and 0.2 m...

Chlorophytum arundinaceum is an important medicinal plant and its tuberous roots are used for various health ailment treatments. It has become an endangered species in the Eastern Ghats, and a rare medicinal herb in India, due to its excessive collection from its natural habitat and its destructive harvesting techniques, coupled with poor seed germination and low vegetative multiplication ratio...

This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·Lsucrose, 5.5 g·Lagar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·LTDZ, 0.1 mg·LNAA supported the maximal auxil...

In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock, was achieved through terminal/axillary bud culture using Murashige and Skoog (MS) basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and 0.1 mg/L indole-3-butyric acid (IBA). Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival...

A complete protocol for micropropagation of Musa sapientum using shoot meristems was developed. Multiple shoots were induced in vitro from shoot meristems. Murashige and Skoog’s medium supplemented with BAP and NAA (3.0 + 0.2 mg/l, respectively) was found to be most suitable combination. Further multiplication of shoots required habituation of cultures up to 3 passages of 21 days each on the sa...

Momordica dioica Roxb. (Spine Gourd) (Cucurbitaceae) is an important vegetable with high food and medicinal value. Maintaining tuber quality in field conditions as well its conservation during storage is difficult. Micropropagation may help to overcome these problems to a great extent. As there is limited information on the above mentioned aspects is not available. Therefore, the present study ...

Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N6 –benzylaminopur...