The aim of this study is to examine the influence of Sertoli cells on LH binding to Leydig cells in culture in immature mice. Leydig cells and Sertoli cells were obtained from the testes of immature C57BL/6Ncrj mice and were cultured in serum-free medium for 7 days. The LH binding to Leydig cells and the FSH binding to Sertoli cells were dependent on incubation time, the number of cells, and th...

The potent smooth muscle agonist endothelin-1 (ET-1) is involved in the local control of seminiferous tubule contractility, which results in the forward propulsion of tubular fluid and spermatozoa, through its action on peritubular myoid cells. ET-1, known to be produced in the seminiferous epithelium by Sertoli cells, is derived from the inactive intermediate big endothelin-1 (big ET-1) throug...

We have previously reported that Sertoli cells in culture secrete a transferrin-like protein (Skinncr and Griswold, 1980). The purpose of this study was to determine to what extent hormones such as follicle-stimulating hormone (FSH), insulin and testosterone, and vitamin A can regulate the amount of testicular transferrin secreted by cultured Sertoli cells. Sertoli cell culture medium was colle...

Sertoli cell tumors of the testis are extremely rare tumors with a heterogeneous pathology. Three histological variants have been described: Sertoli cell tumor not otherwise specified (NOS), large cell calcifying sertoli cell tumor and the Sclerosing Sertoli cell tumor. The sclerosing Sertoli cell tumor described herein is associated with prominent stromal sclerosis. They present as painless...

Postnatal Sertoli cell maturation is characterized by a pronounced rise in androgen receptor (AR) expression, which increases several fold between birth and adulthood. Since both 3,3',5-triiodothyronine (T3) and FSH regulate Sertoli cell proliferation and differentiation, we have determined the effects of T3 and FSH on AR mRNA expression in cultured Sertoli cells from 5-day-old rats. These cult...

BACKGROUND Peutz-Jeghers syndrome (PJS) is a rare autosomal-dominant disorder characterized by multiple gastrointestinal hamartomatous polyps, mucocutaneous pigmentation and increased predisposition to various neoplasms. Endocrine manifestations in PJS include gynecomastia due to calcified Sertoli cell testicular tumors usually referred to as large-cell calcifying Sertoli cell tumors (LSCT). ...

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent environmental contaminants. Some PAHs are carcinogens and may affect the male reproductive system. Therefore, we exposed cultured rat Sertoli cells to a variety of PAHs to determine possible direct toxic effects on the cells of the seminiferous epithelium. Sertoli cells were chosen because they support germ cell development a...

We examined the synthesis and deposition of extracellular matrix (ECM) components in cultures of Sertoli cells and testicular peritubular cells maintained alone or in contact with each other. Levels of soluble ECM components produced by populations of isolated Sertoli cells and testicular peritubular cells were determined quantitatively by competitive enzyme-linked immunoabsorbent assays, using...

spermatogonial stem cells (sscs) maintain spermatogenesis throughout life in the male. maintenance of sscs and induction of spermiogenesis in vitro may provide a therapeutic strategy to treat male infertility. this study investigated in vitro differentiation of mouse sscs in presence or absence of sertoli cells, hormones and vitamins. spermatogonial populations were enriched from testes of 4-6 ...

objective(s):leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. the present study was designed to explore morphometrical changes of mouse testicular tissue after long-term administration of leptin. materials and methods:thirty healthy mature male mice were randomly assigned to either control (n=15...