proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

conclusions the incidence of mbls producing p. aeruginosa in patients with cf was not low. the findings indicate that the identification of drug-resistance patterns in p. aeruginosa and the detection of mbl-producing isolates are important in the prevention and control of infections. background respiratory infections caused by pseudomonas aeruginosa play an important role in the pathogenesis of...

random peptide libraries (rpl) displayed on the surface of filamentous bacteriophages have been extensively used as a tool to map epitopes or to identify antigenic mimics (mimotpoes) of disease-specific monoclonal antibodies or polyclonal sera. these rpl are engineered by the insertion of degenerate oligonucleotides, encoding a specific number of random amino acids, in frame with a bacteriophag...

the thermodynamic functions such as enthalpy (h), gibbs free energy (g) and entropy (s) of serineand threonine amino acids were theoretically studied at different condition (solvents andtemperatures) by using gussian o3, software. first, the structural optimization of isolated serine andthreonine were done in the gas phase by using the hartree-fock (hf) level of theory with 3-21g, 6-31g and 6-3...

Inclusion body hepatitis (IBH) is considered one of the re-emerging diseases avian virus that causes economic damage worldwide. IBH caused by different serotypes fowl adenovirus (FAdV), and most FAdV cases in Malaysia are related to serotype 8b. The objective this study was determine molecular markers UPMT27 Malaysian isolate identify evolutionary relationship through phylogenetic approach. Pro...

adverse outcome of pregnancy is a potentially dangerous complication of conception and affects 15-20% of pregnancies. according to recent studies increased level of blood antiphospholipids antibodies, if accompanied by predisposing factors, can be an adverse event in human pregnancy. the aim of the present study was to compare the maternal serum levels of anticardiolipin and antiphosphatidyl se...

in heat-stressed broiler chickens. One hundred and twenty chicks were allocated into four groups of 30 each. Group A (feed restriction), B restriction + L-serine), C (ad libitum) D libitum L-serine). Feed (20%) was implemented on days 7–14, L-serine (200 mg/kg) provided orally from 1–14. Seven chickens slaughtered each group at 35 old to determine meat drip loss, cooking loss pH; tibia bone wei...

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

The role of atmospheric aerosols on climate change is one the biggest uncertainties in most global models. Organic have been identified as potential cloud condensation nuclei (CCN), and amino acids are organic molecules that could serve CCN. Amino make up a significant portion total material atmosphere, herein we present systematic study hydration for two common acids, glycine serine. We comput...