and Granulocyte Immunobiology, S’Agaró, Girona, Spain, May 9-12, 1998, p 52 (abstr) 6. Hessner MJ, Curtis BR, Endean DJ, Aster RH: Determination of neutrophil antigen NA gene frequencies in five different ethnic groups by the polymerase chain reaction with sequence-specific primers (PCR-SSP). Transfusion 36:895, 1996 7. Lin M, Chen CC, Wang CL, Lee HL: Frequencies of neutrophilspecific antigens...

AIMS To determine whether the polymerase chain reaction with sequence specific primers (PCR-SSP) can assign HLA-DR type more accurately than serology in a routine hospital laboratory. METHODS The 93 patients currently awaiting kidney transplants have been DR typed by serology over the past 14 years, 82% within the past five years. They have now been retyped using the PCR-SSP method described ...

In this study we report the development of primers to amplify polymorphic chloroplast simple sequence repeats in the genus Hordeum, which includes cultivated barley (H. vulgare ssp. vulgare) and its wild progenitor H. vulgare ssp. spontaneum. Polymorphic products were amplified in a wide range of Hordeum spp. and intraspecific variation was detected in both cultivated and wild barley. A decreas...

Aim.To study the immunogenetic prerequisites of idiopathic recurrent spontaneous pregnancy losses in humans allo- and autoimmune genesis. Methods. PCR-SSP (polymerase chain reaction with sequence-specific primers). Results. A comprehensive analysis distribution frequency allelic variants HLA-DRB1, HLA-DQA1, HLA-DQB1 genes testifies to an increased risk a total homology 50 % or more couples pres...

Staphylococcus saprophyticus surface-associated protein (Ssp) was the first surface protein described for this organism. Ssp-positive strains display a fuzzy layer of surface-associated material in electron micrographs, whereas Ssp-negative strains appear to be smooth. The physiologic function of Ssp, however, has remained elusive. To clone the associated gene, we determined the N-terminal sequ...

Hereditary haemochromatosis is a common genetic disorder that causes hyperabsorption of dietary iron, leading to increased deposition and various organic diseases. Early diagnosis is important if effective treatment is to be applied and the iron overload corrected before the onset of clinical symptoms. Recently, a candidate gene has been identified in which a single point mutation shows a very ...

interferon- gamma (ifn- γ) is an important immune regulator and inflammatory cytokine which is implicated in the pathogenesis of multiple sclerosis (ms). a single nucleotide polymorphism, t to a, at position +874 in the first intron has previously been shown. this polymorphism is associated with ifn- γ production level. to study the effect of this polymorphism on susceptibility to multiple scle...

Transcriptional status of two intra-genomic ITS variants (Type-I and Type-II) identified in Vigna unguiculata ssp. tenuis (NI 1637) was analysed by RT-PCR. Total RNA was isolated from leaves, stem and roots of 15-day old seedlings and was reverse transcribed using specific primers for amplifying ITS-1 and ITS-2. Gel electrophoresis on a high-resolution agarose revealed that only Type-II variant...

Serology and DNA techniques are employed for Human Leukocyte Antigen (HLA) typing in different transplant centers. Results may not always correlate well and may need retyping with different technique. All the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring HLA typing for bone marrow transplant were enrolled in the study. Serological HLA typing was ...