The aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. Three groups of male adult rats were used in this study. The first group was injected by single dose of busulfan (10 mg kg(-1)) and their testes were removed on day 35 post injection. The second group received double doses of busulfan with 21 days interval and their testes were...

The aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. Three groups of male adult rats were used in this study. The first group was injected by single dose of busulfan (10 mg kg-1) and their testes were removed on day 35 post injection. The second group received double doses of busulfan with 21 days interval and their te...

Atrophic changes in the basal portion of the seminiferous tubules caused by daily injections of estradiol have been found by scanning electron microscopy disclosed after a digestion technique using collagenase and trypsin. In the control rat, the smooth basal portion of the epithelium was composed of polygonal Sertoli cells and reticularly-arranged ovoidal spermatogonia. After estradiol treatme...

Background: Busulfan is an alkylating agent, which inhibits cell dividing by sticking to one of the DNA strands. The aim of the present study was establishment and stereological evaluation of testes of azoospermic animal model using busulfan in albino hamster. Materials and Methods: Male adult albino hamsters were randomly assigned into 3 groups. The first group was injected one dose of busulfa...

Objective(s): Adipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration ...

Tubular boundary tissue and seminiferous epithelia were evaluated stereologically in testes from 28 men aged 20 to 48 years and 28 men aged 50 to 90 years. Testes obtained at autopsy within 15 hours of death were perfused with glutaraldehyde, embedded in Epon (Ladd Research Industries, Inc., Burlington, VT), sectioned at 0.5 micron, and stained with toluidine blue. Volume densities (percentage ...

The aim of this study was to establish a quantitative standard for the cellular composition in seminiferous tubules at each stage of spermatogenesis in the mouse testis, and thereby evaluate abnormalities in the infertile mouse testis. We applied a combination of lectin histochemistry for acrosomes and immunohistochemistry for various specific cell markers, both of which were visualized with fl...

The effect of endosulfan, an Organochlorine pesticide, on bluegill testes was studied. Endosulfan is aqua-toxic and has an immediate effect on fish and other aquatic life. In this experiment, we exposed the fish for 24-, 48-, 72-, 96-h, and 1- and 2-week periods. A second group of fish without exposure to endosulfan served as the control. The control testis appeared structurally normal. The sem...