OBJECTIVES To review a large experience with sperm cryopreservation in patients with testicular cancer and determine the effect of clinical stage and tumor histologic features on semen quality. METHODS The prefreeze and post-thaw sperm quality of 157 patients with testicular cancer was compared with that of 50 normal donors. The impact of tumor stage and histologic features (pure seminoma, pu...

The objective of this research was to verify the presence of spermatic abnormalities on semen of Brycon orbignyanus after cryopreservation. Semen was collected from ten four-year-old males who presented secondary reproductive characteristics for migrating fish. Sperm was evaluated for motility, vigor and spermatic morphology before and after cryopreservation. A cryoprotectant solution was made ...

BACKGROUND Although routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men. ...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Semen cryopreservation in domestic birds has been studied extensively in the past fifty years. However, efficient methods to freeze semen of chicken of different breeds have emerged only in the last decade of the 20 century. Methods using cryoprotectants Glycerol, Dimethyl Acetamide and Dimethyl sulfoxide, slow or rapid freezing-thawing procedures, pellets or ampoules or straws packaging have b...

Cryopreservation has the capacity to extend spermatozoa’s lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

Female moths of Bombyx mori were artificially inseminated with cryopreserved semen. The fertility of inseminated females varied from 0% to 76.9% depending on the strain. Addition of fresh semen from triploid males, which are infertile but whose semen includes intact apyrene sperm, greatly improved fecundity of cryopreserved semen from normal males. Frozen apyrene sperm from the triploid donors ...

objective(s):althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm dna fragmentation and infertility. the post-thaw survival of sperm...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...

AIM The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis. MATERIALS AND METHODS Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks inter...