Identical amounts of each protein were analyzed by SDSPAGE and with the Experion system (Bio-Rad Laboratories). For SDS-PAGE, samples were prepared by mixing equal volumes of protein sample and 2x Laemmli sample buffer with or without 5% β-mercaptoethanol. Following separation on CriterionTM Tris-HCl 4–20% precast gels, gels were stained with Bio-SafeTM colloidal Coomassie Blue stain and imaged...

Mesophilic aeromonads are being increasingly reported pathogen of humans and lower vertebrates. Water and foods are considered to be the chief source of Aeromonas spp. At present there are several techniques available for the detection of Aeromonas spp. from water and foods. However, there is still need to develop immunodiagnostics for rapid detection of Aeromonas spp. irrespective of their spe...

Three shrimps population (Penaeus semesulacatus, Penaeus japonicus,Meta Penaeus monoceros) were investigated for determination of the genetic structure by using (PCR-RAPD) and SDS-PAGE. The results obtained from RAPD technique showed the same percentages of similarity (100%) within the three studied population. On the other hand, the degrees of similarity detected by SDSPAGE were 90%, 87% and 8...

Complex I purified from bovine heart mitochondria is a multisubunit membrane-bound assembly. In the past, seven of its subunits were shown to be products of the mitochondrial genome, and 35 nuclear encoded subunits were identified. The complex is L-shaped with one arm in the plane of the membrane and the other lying orthogonal to it in the mitochondrial matrix. With mildly chaotropic detergents...

Exsheathing fluid (EF) and excretory-secretory products (ES) of infective third-stage cultured larvae ofOstertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE). Five and seven predominant proteins were found in the EF and ES products, respectively. Immunoblotting by sheep pre-infection serum did not react with any of the EF and ES proteins, b...

exsheathing fluid (ef) and excretory-secretory products (es) of infective third-stage cultured larvae ofostertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sdspage). five and seven predominant proteins were found in the ef and es products, respectively. immunoblotting by sheep pre-infection serum did not react with any of the ef and es proteins, b...

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

We have previously demonstrated that increasing factor XIII concentrations above that present in plasma (1 U/mI) results in the formation of very high molecular weight a fate polyacrylamide and agarose gel electrophoresis (SDSPAGE). In this report. we have examined the effect of such crosslinking on plasmic susceptibility of fibrin prepared from purified fibrinogen and from plasma in the presen...

the aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.urine samples from 15 heartworm-infected and 15 healthy dogs were taken. urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (u p/c) ratio were determined. urine proteins werefractionated using sds-page. results showed statistically significant differences ...

Inosine diphosphatase (IDPase) isoforms associated with Golgi membranes were studied in sycamore cell culture. These enzyme isoforms were solubilized with Triton X-100 and purified by chromatography using DEAE-Toyopearl and SOURCES columns. The isoforms were separated into two distinguishable fractions (peak I and 2) by SOURCE S column c.hromatography. Furthermore the peak l contained at least ...