Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

the 1rs chromosome segment derived from petkus rye carries genes for resistance to three wheat rust diseases, namely lr26 for resistance to leaf rust (caused by puccinia triticina), yr9 for resistance to stripe rust (p.striiformis f. sp. tritici) and sr31 for resistance to stem rust (p. graminis f. sp. tritici). since sec-1 is tightly linked with the three rust resis-tance genes electrophoresis...

the purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). i...

seed protein usually is not sensitive to environmental fluctuations; its banding pattern is very stable which advocated for cultivars identification purpose in crop. this study was planned to study effect of water deficit on protein content and protein banding pattern of wheat genotypes. in this study five wheat genotypes were planted in early november, growing season of 2008-2009. the experime...

it is not clear what factors determine divergent outcomes of infections caused by h. pylori . in the present study, the protein profiles of different strains of h. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1dsds-page. the patterns of different h. pylori strains were highly divergent. about 30.76% (7 b...

copper is most toxic metal in marine organisms. characterization of protein occurring in the metabolically active tissues of muscle (mu), hepatopancreas (hp) and gills (gl) of the spiny lobster, panulirus homarus homarus on exposure to two sub-lethal doses (9.55 and 19.1 µg/l) of copper were studied for 28 days of exposure (doe). the electrophoretic pattern of muscle, hepatopancreas and gill pr...