The stromal cell-derived factor-1 (SDF-1) is an alpha chemokine that binds to the CXCR4 receptor. Knock-out studies in mice demonstrate that this ligand-receptor pair is essential in hematopoiesis. One function of SDF-1 appears to be the regulation of migration of hematopoietic progenitor cells. We previously characterized signal transduction pathways induced by SDF-1a in human hematopoietic pr...

The human CXC chemokine, stromal cell–derived factor 1 (SDF-1A), is known to function in vitro as a chemotactic factor for lymphocytes, monocytes, and dendritic cells. In the context that dendritic cells are powerful antigen-presenting cells, we hypothesized that adenoviral gene transfer of SDF-1A to tumors might inhibit growth of preexisting tumors through attracting dendritic cells to the tum...

In this study, we have characterized the signaling pathways mediated by CXCR4 in breast cancer cells and its role in breast cancer cell invasion and migration. Stromal cell-derived factor 1A (SDF-1A; CXCL12) stimulation of breast cancer cells resulted in phosphoinositide 3-kinase (PI-3K) activation, AKT phosphorylation, and activation of the FKHRL1 transcription factor. In addition, SDF-1A indu...

CXCR4 is the receptor for the a-chemokine stromal cellderived factor 1 (SDF-1) and has been shown to be expressed on a diversity of leukocytes. In this report, the expression of the CXCR4 receptor in cells of megakaryocytic lineage and the role of SDF-1 in megakaryocytopoiesis were investigated. Using flow cytometry in combination with reverse transcriptase-polymerase chain reaction (RT-PCR), w...

Tumor-associated macrophages promote tumor growth by stimulating angiogenesis and suppressing antitumor immunity. Thus, therapeutics that inhibit macrophage recruitment to tumors may provide new avenues for cancer therapy. In this study, we showed how chemoattractants stromal cell-derived growth factor 1 alpha (SDF-1a) and interleukin 1 beta (IL-1b) collaborate with myeloid cell integrin-a4b1 t...

Tumor-associated macrophages promote tumor growth by stimulating angiogenesis and suppressing antitumor immunity. Thus, therapeutics that inhibit macrophage recruitment to tumors may provide new avenues for cancer therapy. In this study, we showed how chemoattractants stromal cell-derived growth factor 1 alpha (SDF-1a) and interleukin 1 beta (IL-1b) collaborate with myeloid cell integrin-a4b1 t...

Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Guided PGC migration in zebrafish thus serves as a model for chemokinedirected cell migration, a process that is relevant for a wide range of events in development, homeostasis and disease. We show that during their migration PGCs generate bleb-like protrusions that are powe...

Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Guided PGC migration in zebrafish thus serves as a model for chemokinedirected cell migration, a process that is relevant for a wide range of events in development, homeostasis and disease. We show that during their migration PGCs generate bleb-like protrusions that are powe...

Primordial germ cell (PGC) migration in zebrafish is directed by the chemokine SDF-1a that activates its receptor CXCR4b. Guided PGC migration in zebrafish thus serves as a model for chemokinedirected cell migration, a process that is relevant for a wide range of events in development, homeostasis and disease. We show that during their migration PGCs generate bleb-like protrusions that are powe...

Bone marrow cells (BMC) are critical to the expansion of the tumor vessel network that supports Ewing sarcoma growth. BMCsmigrate to the tumor and differentiate into endothelial cells and pericytes.We recently demonstrated that stromal-derived growth factor 1a (SDF-1a) regulates platelet-derived growth factor B (PDGF-B) and that this pathway plays a critical role in bone marrow–derived pericyte...