the influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘bianca’ and ‘el torro’. third, fourth and fifth nodal explants were cultured on the modified ms medium supplemented with 1.0 and 5.0 mg/l ba. in both the cultivars, higher rate of proliferation (‘bianca’ 3.75; ‘el torro’ 2.65) were obtained from the explants distal to the a...

There is accumulating evidence that the mammalian cerebral cortex is regionally specified early in neurogenesis. However, the degree and scale of the regional pattern that is intrinsic to different parts of the cortical primordium remains unclear. Here, we show that detailed patterning-the accurate positioning of several areas or fields-is intrinsic to the part of the primordium that generates ...

Fibrochondrocytes within meniscal tissue have been shown to alter their biochemical activity in response to changes in their mechanical environment. Meniscal tissue is known to contain both spherical (chondrocytic-like) and elliptical (fibroblastic-like) cells. We hypothesize that a cell's mechanical environment is governed by local material properties of the tissue around the cell, the cell mo...

Because dopamine influences arginine vasopressin (AVP) release, the present studies were designed to ascertain the dopamine receptor subtype that potentiates angiotensin II-induced AVP secretion in cultured hypothalamo-neurohypophysial explants. Dopamine (a nonselective D1/D2 agonist), apomorphine (a D2 ≫ D1 agonist), and SKF-38393 (a selective D1 agonist) dose dependently increased AVP secreti...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...

Introduction: A reliable source of cultured keratinocytes is essential as a component of oral mucosa substitutes to treat burns and wounds of the oral and maxillofacial region. Primary monolayer cell cultures have been also extremely helpful in the study of the basic biology and responses to stimuli, of both oral and skin keratinocytes, and many studies have used them. There are two techniques ...

Corresponding Author: Sang Un Park Department of Crop Science, University of Chungnam National, 99 Daehak-Ro, Yuseong-Gu, Daejeon, 305764, Korea Email: [email protected] Abstract: Plant tissue culture has proven to be commercially important for the replication of various plants species, including medicinal and ornamental plants. This technique has been used extensively in research for applicatio...

An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid ...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

cumin (cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in iran. the purpose of this study was to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. for this purpose the cumin seeds (kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on ms basal medium. leaf and hyp...