a total of 400 bovine diarrhoeic fecal specimens were obtained and conventional microbial culture, immunomagnetic separation and multiplex pcr were simultaneously carried out on samples. for detection of salmonella at genus level, inv-a universal primer was selected. in order to identifiing of salmonella typhimurium, specific primers of rfbj, fljb and flic related to gene sequence of o4, h2:1,2...

background and objectives : salmonella is one of the leading causes of food-borne diseases. increasing occurrence of antimicrobial resistance, especially multidrug-resistance, in salmonella serovars is a major public health problem worldwide. this study was carried out to detect class i integrons and antibiotic resistance profiles in clinical isolates of salmonella serovars collected from seven...

background: salmonella enterica serovar typhi, as causative agent of typhoid fever, is one of the most important endemic pathogens. non-typhoidal salmonella serovars, including typhimurium , infantis , and enteritidis are amongst the most prevalent serotypes worldwide and in developing areas such as iran. the aim of this study was to apply a uniplex pcr for rapid detection of salmonella spp., a...

background: typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. aim of current study was to evaluate a shortened time –multiplex pcr for rapid detection of different sal monella enterica serovars. methods: the pcr primers for three target genes tyv, prt and inva were subjected for amplification by pcr. by using sim ple dna ...

background: multidrug resistance in salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain. methods: salmonella strains isolated from poultry samples by biochemical positive and negative tests were subjected to pcr and identified as salmonella enteritidis. for detection and identi...

this survey of salmonella and specifically salmonella enteritidis contamination in broiler flocks carried out in amol as one of the most important poultry breeding centers, used as manure, yolk sac, papers and visceral organs from dead birds. standard cultural methods were used for salmonella isolation. slide agglutination or tube agglutination tests were erformed using salmonella somatic o pol...

Background and aims: Salmonella spp. infect cold-blooded and warm-blooded animals and maycause a worldwide zoonotic disease, salmonellosis, in infected animals. Rodents can be Salmonellacarriers without any signs of salmonellosis. The frequency of salmonella contamination, the presence ofvirulence genes (SpvR and SpvB) and antibiotic resistance pattern of isolated Salmonella w...