The plasma high-density lipoproteins (HDL, rho 1.085-1.21 g/ml) of rainbow trout (Salmo gairdneri) have been shown to contain large amounts of apolipoprotein (apo) AI (Mr 28,000) and several other apolipoproteins of Mr less than 14,000 (apo C-like) and of Mr 37,000-38,000, 44,000-45,000 and 53,000-54,000. Comparison of apo AI from trout and human HDL shows them to be similar in Mr and to have s...

Ocular oxygen concentration by the process of counter current multiplication in rainbow trout (Salmo gairdneri) was rapidly suppressed after intraperitoneal injections of the carbonic anhydrase inhibitor CL-11,366. The rapidity with which this drug acted suggested a short circuiting of the choroidal rete mirabile. A comparison was made between the time after injection of inhibitor at which oxyg...

Electron microscopy, together with quantitation using a tracing device linked to a digital computer, reveals that the red and white muscle fibers of Salmo gairdneri differ in diameter, organization of myofibrils, dimensions of myofilaments, volumes and surface areas of T system and sarcoplasmic reticulum, morphology of mitochondria, and content of mitochondria, lipid, and glycogen. Biochemical ...

Laser-Raman and infrared spectroscopic studies reveal abundant beta-pleated sheet conformation in the eggshell proteins of the fish Salmo gairdneri. This secondary structure is the underlying molecular conformation, dictating the formation of the helicoidal architecture of the eggshell. Disulphide bonds crosslink the eggshell proteins of the fertilized eggs and are apparently found in g-g-g (ga...

A preparation was developed to perfuse the coronary circulation in working hearts from rainbow trout (Salmo gairdneri Richardson). The preparation was used to examine pressure-flow relationships for the coronary circulation as the heart generated physiological and subphysiological work loads. Coronary vascular resistance increased exponentially as coronary flow rate decreased. Coronary resistan...

Measurements were made of the free and total calcium concentrations ([Ca] and Cat), acid-base status, haemoglobin concentration, haematocrit and plasma protein concentration in the blood of the rainbow trout, Salmo gairdneri. In isolated blood, [Ca] was proportional to Cat while log[Ca] was inversely proportional to pH. Similar findings have been reported from human blood. During intense activi...

We investigated the pH of interlamellar water of trout (Salmo gairdneri) by following changes in the downstream pH of expired water using a stopped-flow method. As water flowed over the gills of control fish, there was a significant decrease in water pH. Acetazolamide added to the water increased the CO2 disequilibrium, while carbonic anhydrase (CA) eliminated the CO2 disequilibrium relative to...