The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...

background and purpose: microorganism-based synthesis of nanostructures has recently been noted as a green method for the sustainable development of nanotechnology. nowadays, there have been numerous studies on the emerging resistant pathogenic bacteria and fungal isolates, the probable inability of bacteria and fungi to develop resistance against silver nanoparticles’ (snps) antibacterial, ant...

in this study, phenolic compounds of evening primrose leaves (oenothera biennis) were extracted with aceton 70%, ethanol 70% and methanol 70%. total phenolic content of aceton extract was significantly higher than the ethanolic and methanolic extracts. extracts were tested for their antimicrobial activity against lactobacillis plantarum and saccharomyces cerevisiae, using micro broth dilution m...

this study was conducted to evaluate the effect of prebiotic short saccharomyces cerevisiae yeast cell wall (ycw) on growth , nutrition and survival rates , blood indices of rainbow trout (oncorhynchus mykiss) were studied . 600 pieces of salmon with an average weight of (1 ± 20) g 4 treatments and 3 replications (each replication 50 pieces of fish) groups. treatments 1, 2, 3 and 4, respectivel...

A new inducible yeast expression vector, pXS7, was constructed by using the promoter and terminator sequences from the Saccharomyces cerevisiae SOR1 gene, which codes for the sorbitol dehydrogenase protein. We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol.

introduction: the purpose of this study was formulation and preparation of a proper culture medium for saccharomyces cerevisiae var. boulardii with molasses and animal serum. methods: a fully-crossed factorial design contain 5%, 10% and 20% of molasses (m) with 0, 1% and 5% animal serum (s) was used in this study. the ph of all culture medias were adjusted to 5.6 with acetic acid. the seed was ...