The stress-responding protein, GADD45α, plays important roles in cell cycle checkpoint, DNA repair and apoptosis. In our recent study, we demonstrate that GADD45α undergoes a dynamic ubiquitination and degradation in vivo, which process can be blocked by the cytotoxic reagent, arsenite, resulting in GADD45α accumulation to activate JNKs cell death pathway, thereby revealing a novel mechanism fo...

Yes. RNA-seq can be used to quantify transcript levels from a sample. In order to perform useful statistics, one sample is insufficient. Replicates must be used to appropriately power such statistics. The RNA-seq method is an impressive advancement with many applications for studying RNA biology but it does not eliminate biological variability. If the input samples are heavily degraded or have ...

Acidovorax sp. strain MR-S7 was isolated from activated sludge in a treatment system for wastewater containing β-lactam antibiotic pollutants. Strain MR-S7 demonstrates multidrug resistance for various types of β-lactam antibiotics at high levels of MIC. The draft genome sequence clarified that strain MR-S7 harbors unique β-lactamase genes.

bluetongue is an infectious disease that primarily affects sheep. but due to serious socioeconomic consequence of it outbreaks on the international trade it has been included in the oie notifiable diseases (list a). during 2007-8, total number of 130 blood samples gathered from suspected sheep to bluetongue disease in seropositive region including khuzestan, kurdistan, fars, ilam and qum provin...

We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others...

Two recently published but independently derived structures, namely the X-ray crystallographic structure of ribosomal protein S7 and the "binding pocket" for this protein in a three-dimensional model of the 16S rRNA, have been correlated with one another. The known rRNA-protein interactions for S7 include a minimum binding site, a number of footprint sites, and two RNA-protein crosslink sites o...

In the paper we investigate Birkhoff’s conditions (Bi) and (Bi∗). We prove that a discrete lattice L satisfies the condition (Bi) (the condition (Bi∗)) if and only if L is a 4-cell lattice not containing a cover-preserving sublattice isomorphic to the lattice S∗ 7 (the lattice S7). As a corollary we obtain a well known result of J. Jakub́ık from [6]. Furthermore, lattices S7 and S ∗ 7 are consid...

Bacterial ribosomal protein S7 initiates the folding of the 3' major domain of 16S ribosomal RNA by binding to its lower half. The X-ray structure of protein S7 from thermophilic bacteria was recently solved and found to be a modular structure, consisting of an alpha-helical domain with a beta-ribbon extension. To gain further insights into its interaction with rRNA, we cloned the S7 gene from ...

Experimental Details S3 General Considerations S3 Synthesis of 2 S3 Synthesis of 3 S4 Synthesis of 4 S4 Synthesis of 5 S5 Synthesis of 6 S6 Synthesis of 6-HD S6 Synthesis of 7 S6 Synthesis of 8 S7 Reaction of 8 with D2 S7 Reactivity with AB Analogs S7 Time Resolved AB Dehydrogenation Experiments S8 NH2BH2 Trapping Experiments S8 Eudiometric Measurement Details S8 Figure S1—Eudiometer Diagram S9...