the hptlc analysis, antioxidant, and antigout activity of asparagus racemosus, withania somnifera, vitex negundo, plumbago zeylanica, butea monosperma and tephrosia purpurea extracts were investigated. the chemical fingerprinting were carried out by high performance thin layer chromatography (hptlc), antioxidant activity by abts, dpph, frap radical scavenging assays, and antiogout activity by c...

tyrosinase (ec: 1.14.18.1) is a copper - containing enzyme which is distributed in all domains of life such as prokaryote, eukaryote, mammals, invertebrates and plants. tyrosinase catalyzes the oxidation of monophenols to diphenols and diphenols to o-quinones . the tyrosinase crystallographic data shows two histidine -rich regions named cua and cub. a loop containing residues m374, s375 and v37...

flavonoids could serve as potent inhibitors of xanthine oxidase (xo). in the present study, the effects of ruta graveolens l. extract and its major isolated flavonoids, quercetin and rutin, on guinea pig liver xo have been investigated. the inhibitory effects of r. graveolens, quercetin and its glycoside form, rutin, were assayed spectrophotometrically. r. graveolens extract showed moderate inh...

the purpose of the given work was to study the dairy efficiency of the delivered, local and crossbreed goats in three different generations (f1, f2 and f3), derived from their crosses, as well as the biochemical composition of milk and some bloodplasma hormones, participating in the process of lactopoez. for the biochemical tests of milk we have selected casein and serumproteins, iron in lactof...

Accumulating evidence indicates that aldosterone plays a critical role in the mediation of oxidative stress and vascular damage. NADPH oxidase has been recognized as a major source of oxidative stress in vasculature. However, the relation between NADPH oxidase in aldosterone-mediated oxidative stress in endothelial cells remains to be ascertained. The present study aimed to investigate the rel...

the electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (mwcnts) is presented. cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (ppo). a redox couple with an anodic and counterpart cathodic peak is obtained. the influence of various parameters such as ph, scan rat...

Accumulating evidence indicates that aldosterone plays a critical role in the mediation of oxidative stress and vascular damage. NADPH oxidase has been recognized as a major source of oxidative stress in vasculature. However, the relation between NADPH oxidase in aldosterone-mediated oxidative stress in endothelial cells remains to be ascertained. The present study aimed to investigate the rel...

choline oxidase (chox) was chosen as a model enzyme for evaluating the performance of cnts’ functional groups for development of enzyme electrodes. cnts were functionalized with carboxylic acid, amine or amide groups. carboxylic acid, amine and amide functionalized cnts were obtained by acid treatment, ethylenediamine or tetraethylenepentamine chemically modification and ammonia plasma treatmen...

aldosterone is traditionally viewed as a hormone regulating electrolyte and blood pressure homeostasis. recent studies suggest that aldo can cause microvascular damage, oxidative stress and endothelial dysfunction. however, its exact cellular mechanisms remain obscure. this study was undertaken to examine the effect of aldo on superoxide production in human umbilical artery endothelial cells (h...