The Fox-1 RNA recognition motif (RRM) domain is an important member of the RRM protein family. We report a 1.8 Å X-ray structure of the free Fox-1 containing six distinct monomers. We use this and the nuclear magnetic resonance (NMR) structure of the Fox-1 protein/RNA complex for molecular dynamics (MD) analyses of the structured hydration. The individual monomers of the X-ray structure show di...

Abnormal glucocorticoid and neurotrophin signaling has been implicated in numerous psychiatric disorders. However, the impact of neurotrophic signaling on glucocorticoid receptor (GR)-dependent gene expression is not understood. We therefore examined the impact of brain-derived neurotrophic factor (BDNF) signaling on GR transcriptional regulatory function by gene expression profiling in primary...

Schaefer, K. E., W. H. J. Douglas, A. A. Messier, M. L. Shea, and P. A. Gohman. 1979. Effect of prolonged exposure to 0.5% CO* on kidney calcification and ultrastructure of lungs. Undersea Biomed. Res. Sub. Suppl.: S155-S161 Guinea pigs were exposed for up to 8 weeks to 0.5% CO», 21% Oj, and balance N,. Control groups of the same age were kept simultaneously in environmental chambers on air. A ...

Insulin has two important functions that relate to overall metabolic homeostasis. The phylogenetically oldest is the maintenance of sufficient energy stores to allow for development, growth, and reproduction. The newer is as a feedback regulator of plasma glucose. The key role of the central nervous system in both functions is reviewed from a personal perspective, and the development of the con...

Fully strained single-crystal metastable Ge1−xSnx layers were grown on Ges001d in order to probe the role of Sn dopant and alloy concentrations sCSn=1310 cm−3 to 6.1 at. % d on surface roughening pathways leading to epitaxial breakdown during low-temperature s155 °Cd molecular-beam epitaxy of compressively strained films. The addition of Sn was found to mediate Ges001d surface morphological evo...

Amyloid-β peptide (Aβ), generated by proteolytic cleavage of the amyloid precursor protein (APP), plays a pivotal role in the pathogenesis of Alzheimer's disease (AD). The key step in the generation of Aβ is cleavage of APP by beta-site APP-cleaving enzyme 1 (BACE1). Levels of BACE1 are increased in vulnerable regions of the AD brain, but the underlying mechanism is unknown. In the present stud...

Natural health products (NHPs) (known as dietary supplements in the United States) are a popular form of self-care, yet many patients do not disclose their use to clinicians. NHP-drug interactions are known to occur and can harm patients and affect the efficacy of conventional treatment. Using the example of an HIV-positive adolescent who had been responding well to antiretroviral therapy but t...

When activated by high NaCl, tonicity-responsive enhancer-binding protein/osmotic response element-binding protein (TonEBP/OREBP) increases transcription of osmoprotective genes. High NaCl activates TonEBP/OREBP by increasing its phosphorylation, nuclear localization, and transactivating activity. In HEK293 cells, mass spectrometry shows phosphorylation of TonEBP/OREBP-S120, -S134, -T135, and -...