This paper describes the use of a quantitative competitive polymerase chain reaction (QC-PCR) assay; using PCR primers to the rRNA locus of rumen fungi and a standard-control DNA including design and validation. In order to test the efficiency of this method for quantifying anaerobic rumen fungi, it has been attempted to evaluate this method in in vitro conditions by comparing with an assay bas...

this study was conducted to compare fungi and whole rumen microorganisms (wrm) digestibility of treated sugarcane pith by fungi and of buffalos and holstein cows. dry matter (dm), neutral and acid detergent fiber (ndf and adf) digestibilities of steam treated sugarcane pith (stp) by fungi and wrm were compared with a two-steps digestion technique, gas production (gp) and specific rumen fungi cu...

the aim of this study was to investigate the fermentation characteristics and cellulolytic enzymes activities of dromedary camels rumen anaerobic fungi in fed with cultivated and pasture forages. hence, the inoculant of rumen anaerobic fungi was prepared by using specific medium and the gas production, digestibility and enzyme activity of rumen fungi were determined on the basis of a 2 × 2 fact...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...

this experiment was conducted to determine the effect of procceesing of sugarcane pith with high pressure steam (210-220c◦, at 19 bar, over 3 min, on 70 % moisture) on fermentative activity and gas production parameters and degradation by pure microorganism of rumen. the microbial different groups of rumen were separated by physical and chemical methods and using antibiotics. these microbial gr...

Anaerobic fungi were isolated from both the rumen and faeces of nine sheep and a cow. A reliable and simple method for the isolation of anaerobic fungi using 24 h rumen incubated milled straw as the inoculum source was developed. We also evaluate the use of chitin measurements as an assay of rumen fungal biomass. Chitin levels were determined from various sample sources (milled barley straw use...

We present a new perspective for the role of Termitomyces fungi in the mutualism with fungus-growing termites. According to the predominant view, this mutualism is as an example of agriculture with termites as farmers of a domesticated fungus crop, which is used for degradation of plant-material and production of fungal biomass. However, a detailed study of the literature indicates that the ter...

The status of anaerobic zoosporic (Chytridiomycota) fungi along the entire digestive tract of sheep was assessed both analytically and microscopically. Digest samples were taken from different segments of the digestive tracts of three newly killed sheep that previously had been used in experimental dietary studies. These digest samples were tested for the presence of rumen fungi by assessing th...