Degradation of plant N in the rumen results in the formation of ammonia-N and NAN. Rumen bacteria utilise these breakdown products to synthesise their cell proteins. This study was carried out to investigate the in vivo degradation of N-labelled duckweed in the rumen and the utilisation of the degradation products by rumen bacteria. We found that duckweed protein was relatively resistant to rum...

Urea, a non-protein nitrogen for dairy cows, is rapidly hydrolyzed to ammonia by urease produced by ureolytic bacteria in the rumen, and the ammonia is used as nitrogen for rumen bacterial growth. However, there is limited knowledge with regard to the ureolytic bacteria community in the rumen. To explore the ruminal ureolytic bacterial community, urea, or acetohydroxamic acid (AHA, an inhibitor...

The rumen microbiome is integral to efficient production in cattle and shows strong host specificity, yet little is known about what host factors shape rumen microbial composition. Secretory immunoglobulin A (SIgA) is produced in large amounts in the saliva, can coat both commensal and pathogenic microbes within the gut, and presents a plausible mechanism of host specificity. However, the role ...

The aim of this study was to evaluate the effects of adding sulfuric acid to sugarcane tops silage on rumen bacteria and whole rumen microorganisms (WRM) and compare the digestibility of sugarcane tops treated with different amount of urea, molasses and sulfuric acid between Holstein cow and Khouzestan buffalo. Regardless of the type of the treatment, potential of gas production (B) by cow WRM ...

Examination of particulate feed that had been digested in vivo in the rumen, and of the leaves of specific legumes that had been digested in vitro by a mixed population of rumen bacteria, showed that very extensive glycocalyx-enclosed bacterial microcolonies developed on many of the available surfaces. Some of these adherent bacteria colonized a surface almost exclusively and attracted another ...

Anaerobic fungi inhabit the rumen and actively degrade plant cell walls. Rumen fungi produce high levels of cellulases and hemicellulases and are particularly proficient in producing xylanases. These enzymes are regulated by substrate (especially soluble sugars) available to the organisms. Fungi degrade unlignified (i.e., no histochemical reaction for phenolics) plant walls totally, indicating ...

In recent years there has been an increased interest in bacteriocins, primarily among the lactic acid bacteria, as a result of their applications in food preservation. There has been very little research on the occurrence of bacteriocin production among anaerobic bacteria. Recently, we reported a high incidence of bacteriocin-like inhibitory activity in isolates of the rumen anaerobe Butyrivibr...

Total (with one peak) and separate three stereoisomers of 2,6-diaminopimelic acid (DAP) in hydrolysed bacterial species and mixed rumen bacteria were determined. The contents of the total DAP of the hydrolysed mixed rumen bacteria collected from sheep and cattle on the average were 30.1 and 12.3 μmol g bacterial DM . The contents of mesoand LLDAP in the hydrolysed mixed rumen bacteria collected...

Cultures of Streptococcus bovis and mixed populations of rumen bacteria were used to investigate the concentration of ATP and rumen bacterial numbers at various stages of growth. ATP, extracted with Tris buffer, was analyzed using the firefly luciferin-luciferase bioluminescent reaction. ATP concentrations of S. bovis and mixed cultures of rumen bacteria significantly correlated with live cell ...

Five total mixed rations prepared from finger millet (Eleusine Coracana) straw as a roughage (48%) and mixed concentrate (52%), supplemented with a 1% isoacid mixture (i-C4, i-C5, C5 and phenylacetic acid in equal proportions) or oil (groundnut oil, 5% more than the control) or urea (5% more nitrogen than the control), and protein (groundnut cake, 5% more nitrogen than the control) were given i...