نتایج جستجو برای: rpos

تعداد نتایج: 1053  

2013
Sang A Oh Ji Soo Kim Ju Yeon Park Song Hee Han Christian Dimkpa Anne J. Anderson Young Cheol Kim

The stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentially regulated proteins had unknown roles. Changes in levels of proteins in P. chlororaphis O6 rpoS ...

1999
GISELA BECKER EBERHARD KLAUCK REGINE HENGGE-ARONIS

The degradation of the RpoS (sS) subunit of RNA polymerase in Escherichia coli is a prime example of regulated proteolysis in prokaryotes. RpoS turnover depends on ClpXP protease, the response regulator RssB, and a hitherto uncharacterized ‘‘turnover element’’ within RpoS itself. Here we localize the turnover element to a small element (around the crucial amino acid lysine-173) directly downstr...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2015
Aurelia Battesti Nadim Majdalani Susan Gottesman

RpoS, the stationary phase/stress sigma factor of Escherichia coli, regulates a large cohort of genes important for the cell to deal with suboptimal conditions. Its level increases quickly in the cell in response to many stresses and returns to low levels when growth resumes. Increased RpoS results from increased translation and decreased RpoS degradation. Translation is positively regulated by...

Journal: :Journal of bacteriology 2010
Kimberly A M Storvik Patricia L Foster

RpoS, Escherichia coli's general stress response sigma factor, regulates error-prone DNA polymerase IV (Pol IV) (encoded by the dinB gene). Pol IV is induced in stationary-phase cells, and thereafter, levels of the protein remain elevated for several days of continuous incubation. This induction and persistence in stationary-phase cells are dependent on RpoS. Data presented here show that this ...

Journal: :Annual review of microbiology 2011
Aurelia Battesti Nadim Majdalani Susan Gottesman

Under conditions of nutrient deprivation or stress, or as cells enter stationary phase, Escherichia coli and related bacteria increase the accumulation of RpoS, a specialized sigma factor. RpoS-dependent gene expression leads to general stress resistance of cells. During rapid growth, RpoS translation is inhibited and any RpoS protein that is synthesized is rapidly degraded. The complex transit...

2017
Tadayuki Iwase Takashi Matsuo Saiko Nishioka Akiko Tajima Yoshimitsu Mizunoe

RpoS is a key stress-inducible sigma factor that regulates stress resistance genes in Escherichia coli, such as the katE gene encoding catalase HPII and the glg genes encoding glycogen synthesis proteins. Monitoring RpoS activity can provide information on the stress sensitivity of E. coli isolates in clinical settings because the RpoS in these isolates is often mutated. In the present study, w...

Journal: :journal of sciences islamic republic of iran 0

a one kb portion of the rpos gene from flexibacter chinensis was isolated by pcr, sequenced and compared to the rpos gene of a variety of other organisms. the gene was found to be 98% similar to previously sequenced genes. mutation of the rpos gene with tri-parental mating produced strain jr101 and the growth rate of the mutant was compared with that of the wild-type. the mutant grew slower, an...

Journal: :Applied and environmental microbiology 2006
Arvind A Bhagwat Jasmine Tan Manan Sharma Mahendra Kothary Sharon Low Ben D Tall Medha Bhagwat

The stationary-phase sigma factor (RpoS) regulates many cellular responses to environmental stress conditions such as heat, acid, and alkali shocks. On the other hand, mutations at the rpoS locus have frequently been detected among pathogenic as well as commensal strains of Escherichia coli. The objective of this study was to perform a functional analysis of the RpoS-mediated stress responses o...

Journal: :Journal of bacteriology 1999
G Paesold M Krause

In Salmonella dublin, rpoS encodes an alternative sigma factor of the RNA polymerase that activates a variety of stationary-phase-induced genes, including some virulence-associated genes. In this work, we studied the regulation and transcriptional organization of rpoS during growth. We found two transcripts, 2.3 and 1.6 kb in length, that represent the complete rpoS sequence. The 2.3-kb transcr...

Journal: :Journal of bacteriology 2004
Somalinga R V Vijayakumar Mark G Kirchhof Cheryl L Patten Herb E Schellhorn

RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differential expression of an operon-lacZ fusion bank in rpoS mutant and wild-type bac...

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