The Silybum marianum (L.) Gaertn. is the dicotyledonous herbs of the Asteraceae family that is important in medical industry. The biological active compound of S. marianum is a mixture of several flavonolignals generally known as silymarin. The purpose of this study was to optimize S. marianum tissue culture for callus induction, silymarin production and comparison of some biochemical traits be...

This study evaluated the induction of hairy roots in Perovskia abrotanoides Karel, a medicinally important member of Lamiaceae family, using different strains of Agrobacterium rhizogenes and various carbon supplies, for the first time. This research established an efficient Agrobacterium mediated transformation system for this plant species through bacterial strains including ATCC15834, TR105 a...

In Zea mays L., addition of glucose to the induction medium has no effect on the induction of nitrate reductase during the initial 3 hours either in root tips (0-10 mm) or mature root sections (25-35 mm). With longer times, higher levels of enzyme activity are recovered from both root segments when glucose is present in the incubation medium. The induction in root tips is saturated by 10 mm NO(...

Following in vitro infection with Agrobacterium rhizogenes wild strain (mannopine, 8196) and two A. tumefaciens transconjugant strains (C58C1 pArA4abc and C58C1 pArA4b) transformed (hairy) roots were induced in pumpkin (C. pepo L.) cotyledons. The presence of pRi T-DNA in pumpkin long-term hairy root cultures was determined by Southern hybridization. The influence of plant growth regulators on ...

High frequency plant regeneration in A. longifolia (L.) was achieved from leaf explant implanted on MS basal medium supplemented with NAA (0.5 mg/l) + BA (2.0 mg/l) through intervening callus phase. Well-developed shoots (>3cm) were successfully rooted on MS medium supplemented with NAA (0.1 mg/l). Protein and total soluble sugar contents were maximum during organogenesis and multiple shoot ind...

abstract pythium aphanidermatum is one of the most important agent of root and foot rot of cucumber, particularly in the warm humid climate. induction of host resistance by microorganisms or by the synthetic compounds is recently considered as one of the biological control method of disease. trichoderma harzianum bi is a good candidate for induction of resistance in plant against diseases. the ...