this research was carried out to investigate the effects of various levels of dietary organic selenium supplementation (0, 0.1,  0.2 and  0.3 mg/kg) on reproductive traits of rooster. semen was collected using abdominal massage and samples were mixed together. sperm characteristics including percentage of motile, viable and abnormal sperms were assessed. this experiment was carried out based on...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...

The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster (Gallus domesticus ) and Guinea fowl (Numida meleagris ) as model avian species. Seventy-two ejaculates (36 per species studied) wer...

effect of propylthiouracil (ptu)-induced hypothyroidism during the prepubertal period, at a dose of oneg per kg of the diet, on serum testosterone level and seminal characteristics of fars indigenous chickens wasstudied. ptu was fed between 7th to 13th weeks of age and semen was collected at weekly intervals, starting at 21st week of age and continued for seven weeks. the effect of ptu treatmen...

Background: Exposure of sperm membranes to Reactive Oxygen Species induces lipid peroxidation causing membrane breakdown and loss of function. A rosemary extract as dietary additive was assayed for antioxidant effectiveness to improve the quality of rooster semen. Although rosemary extract has been evaluated for in vitro study of semen preservation, up to date no study has been conducted to exa...

background: single domain antibodies from camel heavy chain antibodies (vhh or nanobody), are advantages due to higher solubility, stability, high homology with human antibody, lower immunogenicity and low molecular weight. these criteria make them candidates for production of engineered antibody fragments particularly in transgenic animals. objective: to study the development of transgenic chi...

BACKGROUND Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the p...

This study aimed to determine the effect of method giving rations during day and night on physiological response (respiratory rate, rectal temperature, heart rate) medium type roosters in postal cages best ration methods rooster cage. used a Completely Randomized Design (CRD), consisting three treatments with six replications, those were P1: 30% 70% ration; P2: 50% P3: ration. The chicken was m...

Experiments were designed to determine when, during the cryopreservation process, sperm lose fertilizing capacity and whether the cryoprotectant, methyl cellulose (MC), could be used in combination with glycerol to cryopreserve sperm and remain in the inseminate without reducing fertility. Semen diluted in Minnesota Avian extender (MNA) and inseminated immediately had greater fertility (75%) th...