Transducin is a multi-subunit guanine-nucleotide-binding protein that mediates signal coupling between rhodopsin and cyclic GMP phosphodiesterase in retinal rod outer segments. Whereas the T alpha subunit of transducin binds guanine nucleotides and is the activator of the phosphodiesterase, the T beta gamma subunit may function to link physically T alpha with photolysed rhodopsin. In order to d...

Theoretical electronic Structure methods have been employed to study the structure and activity of certain (free) quinolones and the interaction of their Cu(II)-complexes on a DNA model (Rhodamine 6G (rhod)). As a manner of assessing the generated geometries, the nalidixic acid geometrical parameters obtained were tested against the crystallographic ones and it was found that the average error ...

We introduce and study a new model for functional data. The ARHD is an autoregressive model in which the first order derivative of the random curves appears explicitely. Convergent estimates are obtained through an original double penalization method. The prediction method is applied to a real set of data already studied in the literature.

arly endosomes may help to create their own short-range tracks, according to a report from Stéphane Gasman, Yannis Kalaidzidis, and Marino Zerial (Max Planck Institute, Dresden, Germany). The results also suggest parallels between membrane trafficking, signaling, and regulation of the cytoskeleton. Trafficking of early endosomes requires the small GTPase RhoD. Zerial’s group identified the firs...

Previously, we reported that both the bradykinin (Bk)-induced increase in mitochondrial ATP concentration ([ATP]M) and the rate of cytosolic Ca2+ removal are significantly decreased in skin fibroblasts from a patient with an isolated complex I deficiency. Here we demonstrate that the mitochondrial Ca2+ indicator rhod-2 can be used to selectively buffer the Bk-induced increase in mitochondrial C...

arly endosomes may help to create their own short-range tracks, according to a report from Stéphane Gasman, Yannis Kalaidzidis, and Marino Zerial (Max Planck Institute, Dresden, Germany). The results also suggest parallels between membrane trafficking, signaling, and regulation of the cytoskeleton. Trafficking of early endosomes requires the small GTPase RhoD. Zerial’s group identified the firs...

arly endosomes may help to create their own short-range tracks, according to a report from Stéphane Gasman, Yannis Kalaidzidis, and Marino Zerial (Max Planck Institute, Dresden, Germany). The results also suggest parallels between membrane trafficking, signaling, and regulation of the cytoskeleton. Trafficking of early endosomes requires the small GTPase RhoD. Zerial’s group identified the firs...

Src activation is a tightly regulated process which requires RhoB endosome-associated actin assembly and transit to the cell periphery. We show here that although two other ubiquitous Src family kinases (SFKs) Yes and Fyn also require intact actin filaments for peripheral membrane targeting, they display distinct spatial activation and endosomal requirements. Unlike Src, both Yes and Fyn are co...

The self-diffusion coefficient D for super- and subcritical water is determined by using the proton pulsed-field-gradient spin echo method at high temperatures and low densities. The density of water is ranged in the steamlike region from 0.0041 to 0.0564 g [corrected] cm(-3) at a supercritical temperature of 400 degrees C, also at 0.0041-0.0079 and 0.0041-0.0462 g [corrected] cm(-3) (the steam...