Purpose: Screening scale studies were performed with eight cultures for their ability to metabolize phenobarbital, an antiepileptic, sedative, hypnotic and substrate for CYP 2C9 and 2C19. Methods: The transformation of phenobarbital was confirmed and characterized by fermentation techniques, high performance liquid chromatography (HPLC), mass spectrometry (MS) and metabolism inhibition studies....

highly stabilized monodispersed silver nanoparticles (agnps) were synthesized by rhizopus stolonifer and the antifungal efficacy of silver nanoparticles (agnps) against candida sp. were studied. characterization of biosynthesized nanosilver was made by tem-eds and afm. minimum inhibitory concentration (mic) of biosynthesized agnps, amphotericin b, and fluconazole have been studied on pathogenic...

One of the most economically viable processes for the bioconversion of many lignocellulosic waste is represented by white rot fungi. Phanerochaete chrysosporium is one of the important commercially cultivated fungi which exhibit varying abilities to utilize different lignocellulosic as growth substrate. Examination of the lignocellulolytic enzyme profiles of the two organisms Phanerochaete chry...

Phylogenetic analysis of Rhizopus strains based on the D1/D2 region of LSU rDNA sequences yielded a phylogram with four well-supported clades. The R. microsporus clade concurs with classification obtained by traditional methods. The R. oryzae group was found to include species of the genus Amylomyces. The traditional R. stolonifer group was divided into two well-supported clades in the phylogra...

The growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. Rhizopus stolonifer showed the highest and the fastest utilization of starch and cellulose in the cassava waste substrate. It showed 70% starch utilization and 81% cellulose utilization within eight days. The release of reducing sugars indicating the subst...

Post-harvest treatments of fruits and vegetables can help to reduce the attack microorganisms especially presence pathogenic microorganisms. Alkaline water solutions were used control growth Botrytis cinerea, Rhizopus stolonifer, Salmonella spp. Escherichia coli in strawberry (Fragaria x ananassa). Strawberries inoculated with afterwards immersed alkaline pH 11, 12 13. In vitro microbiological ...

The internal transcribed spacer (ITS) regions including the 3'-end of 18S rRNA gene, 5.8S rRNA gene and the 5'-end of the 28S rRNA gene of Rhizopus spp. were amplified by PCR and analyzed by DNASIS program. Length polymorphism of these region ranged from 564 bp in R. oryzae to 789bp in R. stolonifer. The length and sequence of 5.8S was very conserved with 154~155 bp. The sequence of ITS2 was mo...