نتایج جستجو برای: rh strain

تعداد نتایج: 233256  

Journal: :Journal of medical microbiology 2003
T D Nguyen G Bigaignon D Markine-Goriaynoff H Heremans T N Nguyen G Warnier M Delmee M Warny S F Wolf C Uyttenhove J Van Snick J-P Coutelier

The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection with the T. gondii strains RH and Beverley. High levels of circulating IL12 a...

Journal: :Eukaryotic cell 2009
Asis Khan Michael S Behnke Ildiko R Dunay Michael W White L David Sibley

Toxoplasma gondii has an unusual population structure consisting of three clonal lineages that predominate in North America and Europe. This simple pattern has encouraged the use of only a few laboratory isolates that are representative of each lineage. Principle among these is the type I RH strain, originally isolated from a child with encephalitis some 70 years ago. Comparison of different pa...

A. Dalimi, F. Ghafari Far K. Solhjoo Z. Sharifi

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

2013
Mohey Abdel-Hafez HASSANAIN Eman Hussien ABDEL-RAHMAN Nagwa Ibrahim TOALEB Raafat Mohamed SHAAPAN Hasan Ali ELFADALY Nawal Abdel-Hafez HASSANAIN

BACKGROUND Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurate. To increase the diagnostic potency of antigens, isolation of their immunogenic fractions could be useful. The current research adopted to obtain an affinity isolated fraction from RH strain using CNBr Sepharose 4B column coupled with infected mice sera helping in detection of IgM and I...

Journal: :archives of razi institute 2016
a. dalimi1 f. ghafari far k. solhjoo z. sharifi

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...

2016
Yee-Ling Lau Wenn-Chyau Lee Ranganath Gudimella GuiPing Zhang Xiao-Teng Ching Rozaimi Razali Farhanah Aziz Arif Anwar Mun-Yik Fong

Toxoplasmosis is a widespread parasitic infection by Toxoplasma gondii, a parasite with at least three distinct clonal lineages. This article reports the whole genome sequencing and de novo assembly of T. gondii RH (type I representative strain), as well as genome-wide comparison across major T. gondii lineages. Genomic DNA was extracted from tachyzoites of T. gondii RH strain and its identity ...

Journal: :The Korean journal of parasitology 2003
Jong-Yil Chai Aifen Lin Eun-Hee Shin Myoung-Don Oh Eun-Taek Han Ho-Woo Nan Soon-Hyung Lee

Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from enceph...

2004
M. R. Miner B. Hosticka P. M. Norris

The stress vs. strain relation of hygroscopic aerogels was tested in a controlled humidity chamber to study how the Young s modulus and non-recoverable strain are affected by the adsorption of water in the gel. Mass gains over desiccated conditions varied from 4% at 32% relative humidity (RH) to 12% at 70% RH. Above 70% RH, the samples failed catastrophically. The Young s modulus increased from...

2013
Monavar Selseleh MH Modarressi S Shojaee M Mohebali MR Eshraghian Mina Selseleh H Keshavarz

BACKGROUND Toxoplasma gondii is an obligate intracellular parasite that infects humans at high prevalence rates. The virulent RH strain of T. gondii is generally considered to have lost its cyst forming capacity. This study performed to obtain tissue cysts in mice infected with tachyzoites of RH strain treated with sulfadiazine (SDZ). It provides the opportunity to analyze the conversion of tac...

Journal: :Pathobiology : journal of immunopathology, molecular and cellular biology 2007
M Kato F G Claveria Y Maki K Sanda T Tanaka Y Omata H Nagasawa N Suzuki

OBJECTIVES We compared the reactivity of IgG1 and IgG2a antibodies in mouse sera after infection with virulent RH and low-virulent S273 and Beverley strains of Toxoplasma gondii against RH SAG1 recombinant p30 (rp30) and synthetic SAG1 peptides. METHODS Infected mouse serum samples were collected 9 days after infection, and the level of total IgG, IgG1 and IgG2a against the RH SAG1 rp30 prote...

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