We used dual-color in vivo cellular imaging to visualize trafficking, nuclear-cytoplasmic dynamics, and the viability of cancer cells after their injection into the portal vein of mice. For these studies, we used dual-color fluorescent cancer cells that express green fluorescent protein (GFP) linked to histone H2B in the nucleus and retroviral red fluorescent protein (RFP) in the cytoplasm. Hum...

Isoniazid (INH) and Rifampicin (RFP) are widely used in the world for the treatment of tuberculosis, but the hepatotoxicity is a major concern during clinical therapy. Previous studies showed that these drugs induced oxidative stress in liver, and several antioxidants abated this effect. Metallothionein (MT), a member of cysteine-rich protein, has been proposed as a potent antioxidant. This stu...

Clarithromycin (CAM) and rifampicin (RFP) have both been recognized to be effective antibiotic agents against Mycobacterium avium complex (MAC) infection. Rifamycin derivatives including RFP and rifabutin modulate the CAM metabolism by inducing the hepatic cytochrome p-450 3A4. To clarify the effect of RFP on the CAM metabolism, we measured the plasma concentration of CAM and 14-R-hydroxyclarit...

Procurement refers to a process resulting in delivery of goods or services within a set time period. The process includes aspects of purchasing, specifications to be met, and solicitation notifications as in the case of Request For Proposals (RFPs). Typically, such an RFP is described in a verbal ad hoc fashion, in English, with tables and graphs, resulting in imprecise specifications of requir...

BY4741 (wild-type) MATa ura3∆0; leu2∆0; his3∆1; met15∆0 Openbiosystems RD66 VMA5-GFP::HIS3 Openbiosystems RD68 VPH1-GFP::HIS3 Openbiosystems RD146 VMA5-RFP::KANR This study RD157 VMA5-RFP::KANR; VPH1-GFP::HIS3 This study RD67 VMA2-GFP::HIS3 Openbiosystems RD148 VMA5-RFP::KANR This study RD151 VMA5-RFP::KANR pHluorin::URA3 This study RD57 vma5∆::KANR Openbiosystems RD152 vma5∆::KANR; pHluorin::U...

BACKGROUND The treatment of Buruli ulcer (BU) that is caused by Mycobacterium ulcerans, is currently based on a daily administration of rifampin and streptomycin (RIF-STR). A fully oral intermittent regimen would greatly simplify its treatment on the field. METHODOLOGY/PRINCIPAL FINDINGS The objective of this study was to assess the bactericidal and sterilizing activities of intermittent oral...

AIM To study frequency and incremental prognostic value of restrictive filling pattern (RFP) in hypertrophic cardiomyopathy (HCM). METHODS AND RESULTS Eighty-seven consecutive HCM patients (64% men, mean age 45 +/- 19 years) underwent physical and Doppler echocardiographic evaluation at our centre from March 1993 to February 2001. Mean length of follow-up was 96 +/- 54 months. RFP was found i...

We describe a new data format for storing triangular and symmetric matrices called RFP (Rectangular Full Packed). The standard two dimensional arrays of Fortran and C (also known as full format) that are used to store triangular and symmetric matrices waste nearly half the storage space but provide high performance via the use of level 3 BLAS. Standard packed format arrays fully utilize storage...

Fluorescent fusion proteins are exceedingly useful for monitoring protein localization in situ or visualizing protein behavior at the single molecule level. Unfortunately, some proteins are rendered inactive by the fusion. To circumvent this problem, we fused a hyperactive RecA protein (RecA803 protein) to monomeric red fluorescent protein (mRFP1) to produce a functional protein (RecA-RFP) that...

The ret finger protein (rfp) is a member of the B-box zinc finger gene family many of which may function in growth regulation and in the appropriate context become oncogenic. Members of this family are nuclear proteins that possess a characteristic tripartite motif consisting of the RING and B-box zinc binding domains and a coiled-coil domain. The promyelocytic leukemia gene (PML), another B-bo...