-..''Conversion' of absorbance unit into molar amount was tried as to deoxydinucleotide, In order to achieve rhis trial, a reversedphase high-performance liquid chromatographic method with a linear gradient mode was established for separation and quantification of deoxymononucleotides and deoxynucleosides. Selecting 2'-deoxycytidylyl-(3'-5')thymidylic (5') acid as an example of deoxydinucleotid...

You, J., X. Zhao, Y. Suo, Y. Li, H. Wang, and G. Chen, Determination of LongChain Fatty Acids in Bryophyte Plant Extracts by HPLC with Fluorescence Detection and Identification with MS, J. Chromatogr. B 848:283–291, 2007. A sensitive method has been developed for the determination of long-chain fatty acids (LCFAs) (>C20) using 1-[2-(ptoluenesulfonate)-ethyl]-2-phenylimidazole-[4,5-f]-9,10-phena...

A multi-way control system for spun yarn breakage detecting and tow stopping is presented for linen wet spinning frame upgrade in medium-sized businesses of southeastern China. The signals from periodic rotations of spun yarns are picked by piezoelectric sensors. After input protection and multiplexers, the weak voltage signal from gated channel is processed through links including voltage foll...

Benzodiazepines are among the most prescribed drugs for the treatment of a wide spectrum of clinical disorders. They have been used as anticonvulsants, anxiolytics, hypnotics or muscle relaxants with different durations of action. In this paper, the determination of six frequently used benzodiazepines and metabolites: flunitrazepam, oxazepam, alprazolam, chlordiazepoxide, diazepam and desmethyl...

In insects, hydrophobic odorants are transported through the sensillar lymph to receptors on sensory neurons by odorant-binding proteins (OBPs). The beetle Tenebrio molitor, which is a pest of stored grain products, produces a set of 12–14-kDa OBP-like proteins in its hemolymph. The structure of one of these proteins and that of a moth pheromone-binding protein have been solved. Both proteins h...

Background: Palm oil and its constituents have wide applications in food, cosmetics pharmaceutical industries. The tocotrienol-rich fractions of crude palm drawn greater research interest recent years due to their potent health benefits. Therefore, reliable validated analytical methods are essential for the quantification tocotrienols. Objective: This study aimed develop a simple economical RP-...

Proteomic samples such as human serum are an extreme challenge to study due to the large dynamic range of the proteins of interest. The proteins in human serum can range from those considered “high-abundance” proteins, present at 2%–50% of the total protein mass (typically the μg/mL–mg/mL range) to those considered tissue biomarkers present in the pg/mL–ng/mL range. Multidimensional separation ...

Two simple, accurate, sensitive and reproducible methods have been developed and subsequent validated for the determination of Sildenafil citrate (SC) in presence of its impurities “1-methyl-4-nitro-3-n-propyl-5pyrozole Carboxamide (MNC), 4-amino-1-methyl-3-n-propyl-5-pyrazole Carboxamide (AMP), 4-(2-ethoxy benzoyl amino)-1-methyl-3-n-propyl-5-pyrazole Carboxamide (EMC) and 5-(2-ethoxyphenyl)-1...