Garrido JL and Zapata M (1996) Ion-pair reversed-phase high-performance liquid chromatography of algal chlorophylls. Journal of Chromatography A 738: 285. Hodgson DA, Wright SW and Davies N (1997) Mass spectrometry and reverse phase HPLC techniques for the identiRcation of degraded fossil pigments in lake sediments and their application in palaeolimnology. Journal of Paleolimnology 18: 335. Jef...

A highly efficient technique has been developed for the resolution of several sterols that are intermediates in the biosynthesis of cholesterol and that differ only by one carbon-carbon double bond or by one methyl group. The technique described utilizes reverse-phase high-pressue liquid chromatography on a micronBondapak-C18 column with acetonitrile as eluting solvent. This procedure is capabl...

Two strains of Fusarium sporotrichioides and one strain of F. culmorum were shown to produce the mycotoxin moniliformin in rice culture. Identification was by reverse-phase liquid chromatography, thin-layer chromatography, and mass spectrometry.

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Methods of overcoming these problems in reversed-phase (RP) separations, by judicious selection of the column and mobile phase conditions, are discussed....

A retention prediction model was developed for peptides separated in reversed-phase chromatography. The model was utilized to identify and exclude the false positive (FP) peptide identifications obtained via database search. The selected database included human proteins, as well as decoy sequences of random proteins. The FP peptide detection rate was defined either as number of retention time o...

A system which consisted of multidimensional liquid chromatography (Yin-yang MDLC) coupled with mass spectrometry was used for the identification of peptides and phosphopeptides. The multidimensional liquid chromatography combines the strong-cation exchange (SCX), strong-anion exchange (SAX), and reverse-phase methods for the separation. Protein digests were first loaded on an SCX column. The f...

in this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by hplc analysis. at first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (

We describe a procedure for determining cefuroxime concentrations in serum by using high-performance liquid chromatography. The drug is extracted from serum with dimethylformamide and separated from other substances in the extract by reversed-phase chromatography. The ultraviolet (280-nm) absorption of the effluent was monitored and quantitated based on the height of the cefuroxime peak. Intra-...