نتایج جستجو برای: restriction enzyme

تعداد نتایج: 308199  

Journal: :iranian journal of parasitology 0
m mahami-oskouei department of medical parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran a dalimi department of medical parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran m forouzandeh-moghadam department of medical biotechnology, faculty of medical sciences, tarbiat modares university, tehran, iran mb rokni department of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran (crepi), tehran university of medical sciences, tehran, iran

background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...

Journal: Mycologia Iranica 2016

Paecilomyces variotii is one of the most important causal agents of dieback disease in pistachio (Pistacia vera) gardens. The disease affects different parts of the tree, such as branch and trunk. Assessment of genetic structure in different populations of this species will lead to more useful management of pistachio dieback disease. In this study, genetic variation within samples of P. varioti...

Journal: :research in pharmaceutical sciences 0
h mir mohammad sadeghi r rajaei f moazen m rabbani a jafarian-dehkordi

taq dna polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. o-helix is a part of taq polymerase suggested to play an important role in the enzyme fidelity. the influence of asn666 in this helix on the enzyme function has never been investigated, and therefore by...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد علوم دارویی 1392

بررسی بیان ژن cps1در فاز های مختلف رشد : پس ازشمارش تعداد مشخصی از سلول های آسپرژیلوس فومیگاتوس این قارچ درزمان های 6 ساعته،8 ساعته و 24 ساعت ، کشت داده می شود, از نظر میزان بیان ژن مورد نظر در زمان های مختلف بررسی می شود.استخراج rna از آسپرژیلوس فومیگاتوس و ساختن cdna و rt-pcrدر هر یک از ساعت های مذکور جز مراحل کار در نظر گرفته شده است. 2. تکثیر ژن cps1 از قارچ a.fumigatus: ابتدا توالی...

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