Moving objects are often characterized by coherent motion that is distinct from that of the background. This makes motion a very useful feature for segmenting video sequence. Extracting moving objects from videos is important for many applications like surveillance, traffic analysis etc. In this paper a novel and efficient moving object segmentation algorithm is proposed that is based on indepe...

Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host gen...

Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. On the other hand, DNA damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with DNA fragmentation and chromatin abnormalities in human ejaculates is well documented, ...

background: heat shock protein a2 (hspa2) is correlated with sperm maturity and function. therefore, dysfunctional expression of this gene results in abnormal spermatogenesis. on the other hand, dna damage in spermatozoa is considered to be an important cause of male infertility, and the presence of sperm with dna fragmentation and chromatin abnormalities in human ejaculates is well documented,...

Background: Cysteine has been used as additive in cryopreservation extenders. It contains of thiol group which plays an antioxidant role to eliminate reactive oxygen species (ROS) during freeze -thaw procedure. Excessive ROS production cause DNA damage and subsequently reduce sperm fertility. On the other hand, protamine protein have high levels of cysteine which is critical in packaging and co...

background: dna immunization with plasmid dna encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. the use of plasmid dna vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of dna vaccines exist, such as the possibility of integration into the host gen...

Objective: The discovery and development of natural products with potent antioxidant properties has been one of the most interesting and promising approaches in the search for treatment of CNS injuries. The most significant consequence of the oxidative stress is thought to be the DNA modifications, which can become permanent via the formation of mutations and other types of genomic instability ...

Background: Sperm that bypasses natural apoptosis may find their way into semen. In order to avoid the insemination of such sperm during an intracytoplasmic sperm injection (ICSI) treatment, novel sperm selection procedures such as the Zeta procedure have been implemented. Therefore, the aim of this study was to evaluate whether this procedure, in addition to reducing the number of immature or ...

Background Use of cellular phones emitting radiofrequency electromagnetic field (RF-EMF) has been increased exponentially and become a part of everyday life. This study aimed to investigate the effects of in vitro RF-EMF exposure emitted from cellular phones on sperm motility index, sperm DNA fragmentation and seminal clusterin (CLU) gene expression. MaterialsAndMethods In this prospective stud...

High-quality mRNA extraction is essential for gene expression assays. In this study, we developed a rapid method (20 min) named FACTS the of intact RNA from Pseudomonas aeruginosa and compared its performance to established techniques like RNAsnap CiAR. The integrity, yield, purity presence residual genomic DNA were adopted as assessment criteria. Multiple assays integrity applied, including Ag...