background: escherichia coli (e. coli) is the most extensively used host for the production of recombinant proteins. however, most of the eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. reteplase as a highly disulfide-bonded recombinant protein is an example of difficult to express protein in e. coli. methods: in this s...

BACKGROUND AND PURPOSE Historically, the use of tissue plasminogen activator (tPA) thrombolysis in pregnancy has been regarded as relatively contraindicated. Underlying this stance has been the concern over the risk of bleeding complications in both mother and child. SUMMARY OF CASE We report the successful use of intravenous recombinant tPA (rtPA) thrombolysis in a pregnant woman with acute ...

Background:Echogenic liposomes (ELIPs) encapsulate drugs and gas bubbles within lipid vesicles. The destruction of ELIPs in response to MHz and kHz ultrasound waves has been studied previously. Applying ultrasound above a certain threshold causes encapsulated gas bubbles destruct rapidly by fragmentation or more slowly by acoustically driven diffusion. This study compares the d...

We employed a canine model of coronary thrombosis, induced by injection of radioactive blood clot, via a catheter placed in the left anterior descending coronary artery, to compare effects of recombinant tissue plasminogen activator (rtPA) administered intravenously and administered directly into the coronary circulation. A control group did not receive rtPA. Compared with controls, both rtPA r...

RCVS: Reversible Cerebral Vasoconstriction Syndrome; rtPA: recombinant tissue Plasminogen Activator; GCS: Glasgow Coma Scale; CT: Computed Tomography; ICH: Intracerebral Hemorrhage; MRI: Magnetic Resonance Imaging; MRR: MR Renography; ACA: Anterior Cerebral Artery; MIS: Minimally Invasive Surgery; TCD: Transcranial Doppler; ICU: Intensive Care Unit; MISTIE: Minimally Invasive Surgery plus rt-PA...

Abbreviations: ASPECTS: Alberta Stroke Program Early CT Score; CT: Computed Tomography; EIC: Early Ischemic Changes; FKA: First Known Abnormal; IQR: Interquartile Range; IV: Intravenous; LKN: Last Known Normal; mRS: modified Rankin Scale; MRI: Magnetic Resonance Imaging; rtPA: recombinant Tissue plasminogen Activator; SD: Standard Deviation; SICH: Symptomatic Intracerebral Haemorrhage; SITS-MOS...

Recombinant tissue plasminogen activator (rTPA) is one of the main portions of acute ischemic stroke management, but unfortunately has some complications. Myocardial infarction (MI) is a hazardous complication of administration of intravenous rTPA that has been reported recently. A 78-year-old lady was admitted for elective coronary artery bypass graft surgery. On the second day of admission, s...

The use of recombinant tissue plasminogen activator (rtPA) has been the standard of care for treatment of acute ischemic stroke for several years. Studies evaluating the efficacy, safety, and optimal timing of rtPA use are ongoing. Recently, results of new studies led to expansion of the short timeframe from stroke symptom onset in which a patient can receive this treatment. Additionally, more ...

BACKGROUND Critically ill patients frequently develop acute lung injury (ALI). Disturbed alveolar fibrin turnover, a characteristic feature of ALI, is the result of both activation of coagulation and inhibition of fibrinolysis. Nebulized fibrinolytic agents could exert lung-protective effects, via promotion of fibrinolysis as well as anti-inflammation. METHODS Rats were challenged intratrache...

The fibrinolytic capacity of patients with acute myocardial infarction (AMI) is known to be impaired. The primary regulatory element of the fibrinolytic system is plasminogen activator inhibitor (PAI). It has been previously observed that there are 2 peaks in the plasma PAI level of AMI patients at 4h and 16h after thrombolytic therapy with recombinant tissue plasminogen activator (rtPA). Lanot...