Water-soluble low-molecular weight chitosan (LMWC) and chitooligosaccharides (COs) were obtained from chitosan (16% N-acetylation) by depolymerization induced by potassium persulfate under nitrogen atmosphere for 2 h. They were characterized by IR, X-ray, HPLC and (13)C-NMR. Splitting of C3/C5 signals in the latter indicated a newer conformation, and also showed prominence of acetyl groups in L...

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferr...

in this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (aat) with correct post-translational modifications, a protein production system based on the transduction of cho and cos-7 cells using lentiviral vectors was developed. human aat cdna was cloned into a replication-defective lentiviral vector. the transgene aat-jred chimer was transferr...

BACKGROUND Core outcome sets (COS) comprise a minimum set of outcomes that should be measured and reported in all trials for a specific health condition. The COMET (Core Outcome Measures in Effectiveness Trials) Initiative maintains an up to date, publicly accessible online database of published and ongoing COS. An annual systematic review update is an important part of this process. METHODS ...

BACKGROUND We hypothesized that annuloplasty ring implantation alters mitral annular strains in a normal beating ovine heart preparation. METHODS AND RESULTS Sheep had 16 radiopaque markers sewn equally spaced around the mitral annulus. Edwards Cosgrove partial flexible band (COS; n=12), St Jude complete rigid saddle-shaped annuloplasty ring (RSA; n=10), Carpentier-Edwards Physio (PHY; n=11),...

Whereas UDP-glucuronosyltransferase-2B7 is widely distributed in different tissues, it preferentially detoxifies genotoxic 4-OH-estradiol and 4-OH-estrone (4-OHE(1)) with barely detectable 17β-estradiol (E(2)) conversion following expression in COS-1 cells. Consistent with the UDP-glucuronosyltransferase requirement for regulated phosphorylation, we discovered that 2B7 requires Src-dependent ty...

BACKGROUND Current tuberculosis (TB) reporting protocols are insufficient to achieve the goals established by the Stop TB partnership. Some countries have recommended implementation of active case finding program. We assessed the effect of Cough Officer Screening (an active screening system) on the rate of TB detection and health care system delays over the course of four years. METHODS Patie...

On 20-21 May 2015, more than 150 people with an interest in core outcome sets (COS) gathered at the University of Calgary in Alberta, Canada for the fifth meeting of the COMET Initiative. This was the first annual COMET meeting in North America, and the COMET Management Group are grateful to Cochrane Canada for facilitating the meeting and an excellent joint session on the second day. As well a...

abstract leafy vegetables such as lettuce are more susceptible to post harvest losses, thus application desirable treatments can be recommendable. generally, type of packaging, such as modified atmosphere packaging (map) and wrapping can considerably suppress post harvest loss of horticultural products. in this study, the effects of polypropylene film (thickness 30 and 40 micron) wrapping and m...

Above-ground biomass (AGB) is an important indicator for monitoring crop growth and plays a vital role in guiding agricultural management, so it must be determined rapidly nondestructively. The present study investigated the extraction from UAV hyperspectral images of multiple variables, including canopy original spectra (COS), first-derivative (FDS), vegetation indices (VIs), height (CH) to es...