Spectroscopy on two oxygen-insensitive Ni-Fe hydrogenases from Ralstonia eutropha (NAD-reducing, soluble hydrogenase; hydrogen sensor, regulatory hydrogenase) reveals non-standard catalytic behaviour and unique structures of their Ni-Fe cofactors. Possible mechanistic implications are briefly discussed.

Ralstonia eutropha H16 degraded (mobilized) previously accumulated poly(3-hydroxybutyrate) (PHB) in the absence of an exogenous carbon source and used the degradation products for growth and survival. Isolated native PHB granules of mobilized R. eutropha cells released 3-hydroxybutyrate (3HB) at a threefold higher rate than did control granules of nonmobilized bacteria. No 3HB was released by n...

in order to reduce the costs associated with poly-β-hydroxybutyrate production, growth and poly-β-hydroxybutyrate production of ralstonia eutropha were studied in batch culture on different carbon sources. experiments were designed and conducted to not only lower the cost of poly-β-hydroxybutyrate production by using inexpensive substrates, but also to increase poly-β-hydroxybutyrate production...

Conversion of 3-hydroxypropionate (3HP) from 1,3-propanediol (PDO) was improved by expressing dehydratase gene (dhaT) and aldehyde dehydrogenase gene (aldD) of Pseudomonas putida KT2442 under the promoter of phaCAB operon from Ralstonia eutropha H16. Expression of these genes in Aeromonas hydrophila 4AK4 produced up to 21 g/L 3HP in a fermentation process. To synthesize homopolymer poly(3-hydro...

CDC group IV c-2, an environmental gram-negative bacillus recently proposed for inclusion in the genus Ralstonia, has been isolated in several human infections. Biochemical characterization and 16S ribosomal DNA (rDNA) sequencing with phylogenetic analysis were used to characterize eight clinical isolates and four type strains. Other typing tools, such as pulsed-field gel electrophoresis (PFGE)...

The acetoacetyl-CoA reductase and the polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (formerly Alcaligenes eutrophus) were expressed in Escherichia coli, Klebsiella aerogenes, and PHA-negative mutants of R. eutropha and Pseudomonas putida. While expression in E. coli strains resulted in the accumulation of poly(3-hydroxybutyrate) [PHB], strains of R. eutropha, P. putida and K. aero...

although the application of biodegradative genetically engineered micro organisms (gems) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. lux-marked ralstonia eutropha h850lr (formerly alcaligenes eutrophus h850lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...

The glyoxylate bypass genes aceA1 (isocitrate lyase 1, ICL1), aceA2 (isocitrate lyase 2, ICL2) and aceB1 (malate synthase, MS1) of Ralstonia eutropha HF39 were cloned, sequenced and functionally expressed in Escherichia coli. Interposon-mutants of all three genes (DeltaaceA1, DeltaaceA2 and DeltaaceB1) were constructed, and the phenotypes of the respective mutants were investigated. Whereas R. ...

Although the application of biodegradative genetically engineered micro organisms (GEMs) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. Lux-marked Ralstonia eutropha H850Lr (formerly Alcaligenes eutrophus H850Lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...