The efforts in this study were initiated to develop and validate new solid phases for estimation of hormones by radioimmunoassay (RIA). The study argued the successful application of different hydroxyapatites (HAP) as new solid phases for estimation of Alpha fetoprotein (AFP), Thyroid Stimulating hormone (TSH) and Luteinizing hormone (LH) in human serum. Hydroxyapatites have different alkali ea...

A homogeneous enzyme immunoassay (EMIT) for serum thyroxine determination has been evaluated. It was also compared to our routine method, a radioimmunoassay with use of a commercial kit, Tetra-Tab RIA. Within-day precision was very similar for the two methods, the CV ranging from about 2.5% at 150 nmol of thyroxine per liter to about 5.7% at 25 nmol/L. The two methods were compared by running 1...

1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples. 2. No significant difference was found in the results obtained by the methods. Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. 3. Its rapidity and rela...

We compared measurement of plasma nortriptyline by a recently developed radioimmunoassay technique with values obtained by traditional gas chromatography. The coefficient of correlation (r) was 0.98 for 84 samples from 21 controlled patients; for a separate series of 45 samples from 34 patients who were receiving in addition other medication, r was 0.96. Use of radioimmunoassay in the routine d...

A solid-phase radioimmunoassay technique was adapted for the rapid detection and identification of western equine encephalomyelitis virus in clinical specimens.

A monoclonal antibody to alpha-human atrial natriuretic polypeptide (alpha-hANP), KY-ANP-I, has been produced by fusion of a nonproducing mouse myeloma cell line, X63-Ag8.653, with spleen cells from BALB/c mice immunized with synthetic alpha-hANP conjugated to bovine thyroglobulin using the carbodiimide coupling procedure. Hybridomas were screened for antibody production by radioimmunoassay usi...

A sensitive solid-phase radioimmunoassay for detection of antibodies to varicella-zoster virus (VZV) is described. The antigen consisted of a sonically disrupted extract of VZV-infected human embryo cells. 125I-labeled rabbit anti-human immunoglobulin G (IgG) specific for the Fc portion of human IgG was used to detect human IgG bound to viral antigen. With this technique, 193 human sera were ev...

The 125I-radioimmunoassay for methaqualone in human urine was evaluated by a comparison with newly modified gas-liquid chromatographic and thin-layer chromatographic methods. The statistically significant sensitivity value for the radioimmunoassay was at 2 microgram of methaqualone per liter of urine. The coefficient of variation was 2.88 +/- 0.39% interassay and 2.71 +/- 0.16% intraassay. Ther...