results analyzing of collected data resulted in the wrapping up the hepatitis virus detection methods in separate sections including 1) immunological methods such as enzyme immunoassay (eia), radio-immunoassay (ria) immuno-chromatographic assay (ica), and immuno-chemiluminescence 2) molecular approaches including non-amplification and amplification based methods, and finally 3) advanced biosens...

In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture mediawere collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically andincubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h...

Shock is accompanied by generalised splanchnic hypoperfusion, and splanchnic organs like the pancreas can be damaged, as shown in animal experimental models and in humans, by the presence of high plasma concentrations of trypsin and other pancreatic enzymes. In order to design a radioimmunoassay technique (RIA) for the measurement of equine trypsin-like immunoreactivity (TLI) in biological flui...

fifty-seven patients suffering from systemic lupus erythematosus with different clinical features were studied from various aspects of immunological abnormalities, including anti-nuclear antibodies; anti-dna; anti-sm (smith's antigen); and, other extractable nuclear antigens. the tests employed were microhemagglutination, counter immunoelecterphoresis, and radio immunoassay (ria). the compariso...

SAKURADA, T., SAITO, S., YAMAGUCHI, T., YAMAMOTO , M., DEMURA, R., DEMURA, H., FUKUGHI, S., YOSHIDA, K. and TORIKAI, T. Radioinonunoassay of Triiodothyronine. Tohoku J. exp. Med ., 1973, 110 (4), 329-340 Immuniza tion of rabbits with triiodothyronine-methyl ester hydrochloride conjugated to bovine serum albumin resulted in the production of antibody against triiodothyro nine. Using dextran-coat...

A new type of non-isotopic immunoassay, applied to the determination of serum gentamicin, is reported. The method is based on partial quenching of fluorescence observed when fluorescein-labelled gentamicin is bound by anti-gentamicin serum. The fluorescence intensity of the labelled gentamicin in an unseparated immunoassay incubation mixture therefore serves to indicate the extent of binding, w...

Pesticides are used globally for enhancing crop yields. However, their excessive use/misuse, especially in the developing countries, results in widespread food and environmental contamination. The presence of pesticide residues in food and environment has posed a serious threat to human health and caused a great concern. In order to keep human from being affected, analytical and monitoring syst...

Methods From the Nordic JIA database 192 patients with available sera were included. Serum samples were collected at a median time of 4 months (IQR 2-7) after disease onset. Patients were followed prospectively for a median of 97 months (IQR 95-105). At the 8-year follow-up visit, data on remission according to the preliminary criteria of C. Wallace et al as well as joint damage according to JA...