Cyanobacteria, microorganisms performing oxygenic photosynthesis, must adapt their metabolic processes to environmental challenges such as day and night changes. PipX, a unique regulatory protein from cyanobacteria, provides mechanistic link between the signalling PII, widely conserved (in bacteria plants) transducer of carbon/nitrogen/energy richness, transcriptional regulator NtcA, which cont...

MscS (mechanosensitive channel of small conductance) is ubiquitously found among bacteria and plays a major role in avoiding cell lysis upon rapid osmotic downshock. The gating of MscS is modulated by voltage, but little is known about how MscS senses membrane potential. Three arginine residues (Arg-46, Arg-54, and Arg-74) in the transmembrane (TM) domain are possible to respond to voltage judg...

We investigated the proteolytic processing of mouse pro-GHRH [84 amino acids (aa)] by furin, PC1/3, PC2, and PC5/6A. We created six point mutations in the N- and C-terminal cleavage sites, RXXR decreased and RXRXXR decreased, respectively. The following results were obtained after transient transfection/cotransfection and metabolic pulse-chase labeling studies in several neuroendocrine cells. 1...

Dyavanapalli J, Rimmer K, Harper AA. Reactive oxygen species alters the electrophysiological properties and raises [Ca ]i in intracardiac ganglion neurons. Am J Physiol Regul Integr Comp Physiol 299: R42–R54, 2010. First published May 5, 2010; doi:10.1152/ajpregu.00053.2010.—We have investigated the effects of the reactive oxygen species (ROS) donors hydrogen peroxide (H2O2) and tert-butyl hydr...

Jensen, Ellen, Charles E. Wood, and Maureen Keller-Wood. Chronic alterations in ovine maternal corticosteroid levels influence uterine blood flow and placental and fetal growth. Am J Physiol Regul Integr Comp Physiol 288: R54–R61, 2005. First published July 1, 2004; doi:10.1152/ajpregu.00149.2004.—Previous work from this laboratory demonstrated that the elevation of maternal plasma corticostero...

Protein arginine methyltransferases (PRMTs) catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to the guanidinium group of arginine residues in a number of important cell signaling proteins. PRMT1 is the founding member of this family, and its activity appears to be dysregulated in heart disease and cancer. To begin to characterize the catalytic mechanism of this isozyme, we...

Bacterial lipolytic enzymes were originally classified into eight different families defined by Arpigny and Jaeger (families I-VIII). Recently, the discovery of new lipolytic enzymes allowed for extending the original classification to fourteen families (I-XIV). We previously reported that G. thermodenitrificans EstGtA2 (access no. AEN92268) belonged to a novel group of bacterial lipolytic enzy...

This paper deals with several bijections between cladograms and perfect matchings. The first of these is due to Diaconis and Holmes. The second is a modification of the Diaconis-Holmes matching which makes deletion of the largest labeled leaf correspond to gluing together the last two points in the perfect matching. The third is an entirely new encoding of cladograms, first as a bijection with ...