Putrescine is widely used in industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Although engineered Corynebacterium glutamicum has been successfully used to produce high levels of putrescine, the overall cellular physiological and metabolic changes caused by overproduction of putrescine remains unclear. To reveal the transcriptional changes that occur in resp...

The induction of epidermal ornithine decarboxylase (EC 4.1.1.17) (ODC) following topical application of the tumorpromoting agent 12-O-tetradecanoylphorbol-13-acetate (TPA) to mice can be inhibited by topical application of putrescine, the product of the enzyme. The degree of inhibition depended on both the dose and the time of putrescine application; appli cation of 20 jumol of putrescine 2 hr ...

this experiment was designed to study the effect of putrescine on quality of table grapes during storage. table grapes cvs, rishbaba and olhoghi were dipped in putrescine solutions (0, 1 and 2mm) by the method of pressure-infiltration. the results indicated that after 55 day of storage putrescine 1 mm in rishbaba and at 2mm in olhoghi cultivars were the most effective treatments in maintaining ...

Polyamines are ubiquitous biologically active aliphatic cations that are at least transiently available in the soil from decaying organic matter. Our objectives in this study were to characterize polyamine uptake kinetics in Phytophthora sojae zoospores and to quantify endogenous polyamines in hyphae, zoospores, and soybean roots. Zoospores contained 10 times more free putrescine than spermidin...

The metabolisms of arginine (Arg), ornithine (Orn), and putrescine were compared in a nontransgenic and a transgenic cell line of carrot (Daucus carota L.) expressing a mouse Orn decarboxylase cDNA. [14C]Arg, [14C]Orn, and [14C]putrescine were fed to cells and their rates of decarboxylation, uptake, metabolism into polyamines, and incorporation into acid-insoluble material were determined. Tran...

Polyamines are involved in the regulation of some Trichomonas vaginalis virulence factors such as the transcript, proteolytic activity, and cytotoxicity of TvCP65, a cysteine proteinase (CP) involved in the trichomonal cytotoxicity. In this work, we reported the putrescine effect on TvCP39, other CP that also participate in the trichomonal cytotoxicity. Parasites treated with 1,4-diamino-2-buta...

Kim, Ki-han (Wayne State University, Detroit, Mich.). Isolation and properties of a putrescine-degrading mutant of Escherichia coli. J. Bacteriol. 86:320-323. 1963.-A mutant was isolated from Escherchia coli B which can grow on putrescine as the sole source of carbon and nitrogen. This mutant contains a constitutive putrescine alpha-ketoglutarate transaminase and can be induced for the enzymes ...

The transport of putrescine into a moderately salt tolerant cyanobacterium Synechocystis sp. PCC 6803 was characterized by measuring the uptake of radioactively-labeled putrescine. Putrescine transport showed saturation kinetics with an apparent K(m) of 92 +/- 10 microM and V(max) of 0.33 +/- 0.05 nmol/min/mg protein. The transport of putrescine was pH-dependent with highest activity at pH 7.0....