Love, Samuel H. (Bowman Gray School of Medicine, Wake Forest College, Winston-Salem, N.C.), and Charles N. Remy. Metabolism of methylated purines in Escherichia coli: derepression of purine biosynthesis. J. Bacteriol. 91:1037-1049. 1966.-Various methylated purines were examined for their effects on growth of purine-requiring mutants of Escherichia coli, strains W-11 and B-96, and for their effe...

Natural and synthetic purine derivatives such as caffeine, theophylline, 6-mercaptopurine and 8-chlorotheophylline are important drugs. Due to the structural similarity of these compounds, it is intrinsically difficult to prepare chemosensors for their selective optical detection. Here, we describe a sensor array which can be used to differentiate pharmacologically important purine derivatives ...

A purine-sensitive phenotype results from a previously described mutation in the structural gene (pyrE) for orotate phosphoribosyltransferase (OPT) in Escherichia coli K-12. OPT from both the mutant and the wild-type was partially inhibited by adenine and adenosine, although other purine derivatives were not effective for this inhibition. The Km values of the mutant OPT were 580 and 760 microM ...

Microbial protein synthesis in the rumen would be optimized when dietary carbohydrates and proteins have synchronized rates and extent of degradation. The aim of this study was to evaluate the effect of varying ruminal degradation rate of energy and nitrogen sources on intake, nitrogen balance, microbial protein yield, and kinetics of nutrients in the rumen of growing kids. Eight Boer goats (38...

A series of new sulfonamide derivatives, 9-(substitutedbenzenesulfonyl)-6-chloro-9H-purines 7(a-e) and carbamate derivatives, 6-chloro-purine-9-carboxylic acid substituted alkyl/arylester 9(a-d), have been synthesized through an intermediate, sodium salt of 6-chloro-9(H)-purine (6) which was prepared by the treatment of 6-chloro-9(H)-purine (4) with sodium hydride. Structures of the newly synth...

It has been reported that the capacity of rabbit bone marrow to carry out the synthesis de novo of purine ribonucleotides is quite low, and that this tissue requires preformed purine derivatives supplied by the liver (1, 2). Presumably, the salvage enzymes, including nucleoside phosphorylases (3),. participate in the reutilization of the preformed derivatives. So far, only pyrimidine nucleoside...

In addition to the bound purine moieties of DNA and RNA, plants and animals contain a variety of other purine derivatives ranging in decreasing structural complexity from free nucleotides to simple substituted bases. Many of these compounds play important roles in metabolism and in its control and integration. Whereas most of the purine derivatives found in animal tissues also occur in plants, ...

Four derivatives of 2,6-diaminopurine (1) were synthesised and characterised. When 1 was reacted with chloroacetaldehyde, 5-aminoimidazo[2,1-i]purine (2), 9-aminoimidazo[2,1-b]purine (3), 9-aminoimidazo[1,2-a]purine (4) and diimidazo[2,1-b:2',1'-i]purine (5) were formed. The purified products (3-5) were fully characterised by MS, complete NMR assignments as well as fluorescence and UV spectrosc...